Phosphorus (P) and iron (Fe) are essential nutrients for plants. Iron availability is low in calcareous soils of Iran due to the excessive amounts of CaCO3 and high pH. Over-fertilization of P fertilizers may also decrease Fe availability. The objective of this study was to evaluate the effect of P and Fe on the growth and chemical composition of corn (Zea mays L.) under greenhouse conditions. Treatments consisted of a factorial arrangement of P rates (0, 40, 80, 120 and 160 mg kg-1 as KH2PO4) and Fe rates (0, 2.5, 5 and 10mg kg-1 as Fe EDDHA) in a completely randomized design with four replications. Plants were grown for 8 weeks in a loamy soil, classified as Chitgar series (fine-loamy, carbonatic, thermic, Typic Calcixerepts).Results showed that P application up to 80 mg kg-1 increased corn top dry matter. Corn P concentration and total uptake increased by P application but decreased by Fe application. Application of Fe up to 5 mg kg-1 increased dry matter but decreased it at higher rates. Concentration and total uptake of Fe increased by Fe application but decreased by P application. Zinc and copper concentrations decreased significantly when P was added. Manganese concentration increased at 40 mg P kg-1 but decreased at higher rates. Iron application decreased zinc and manganese concentrations but had no effect on copper.