Iron deficiency which usually appears as chlorosis in young leaves, is considered to be one of the most important nutritional disorder in citrus trees, particularly in calcareous soils. This experiment was conducted to study the effect of ethephon 37% (as an ethylene releasing agent) with two precultural roots application levels of 800 and 1600 µl/l and four foliar application levels of 0(control), 200, 800 and 1600 µl/1 on morphological characteristics of roots, iron absorption, chlorophyll concentration, leaf area, leaf iron concentration and iron content per 2individual leaf, leaf fresh and dry weight and specific leaf weight (SLW) of sour lime (Citrus aurantifolia Swing) seedlings. The precultural roots application of 800 and 1600 µl/l ethephon, not only significantly increased roots expansion by auxiliary roots development, but also induced roots hair formation and subapical root swelling. These two levels also considerably increased iron uptake by roots. In this experiment, ethylene increased leaf chlorophyll concentration in two different ways, so that, the precultural roots application level of ethephon enhanced the chlorophyll concentration by increasing iron uptake from soil, while foliar application of ethephon most likely induced chlorophyll concentration by increasing iron uptake in leaves, In this experiment, a better correlation between chlorophyll and Fe in leaves was obtained by calculating the leaf iron content per individual leaf rather than iron concentration per dry weight. However, this index is not very accurate because of SLW variations, especially under the roots treatment by ethephon. The results indicated that in the case of plant fortification with iron, ethylene plays a very important role in root morphology and in the case of iron chlorosis paradox in leaves, it has an important role in root morphology and in the case of iron chlorosis paradox in leaves, it has an important effect on increasing chlorophyll concentration and correcting the chlorosis incidence.