The production of the secondary compounds of phenolic including phenolic acids, lignin, and tannin, acts as a defensive factor against the environmental stress. In this study, three species of Zagros oaks (Q. brantii, Q. infectoria and Q. libani) were examined under drought stress. The seedlings were planted in pots under the same environmental conditions outside. After seedlings reached 4 months of age, the irrigation was stopped until the soil of the pots gradually reached 70% (mild stress), 50% (moderate stress), and 30% (severe stress) of field capacity (Fe) during 15 days. After harvesting the plants and in each stage of the experiment, the total biomass and the amount of the secondary compounds, i.e., total phenol, total tannin, and condensed tannin of the seedlings leaves were measured. The results showed that the water deficit stress significantly decreased the biomass. The results of the secondary compounds for different species showed that only the total tannin of Q. brantii was significantly increased under the mild water stress (70% FC) that was also significantly different from the control. Also, under the severe water deficit stress, the amount of condensed tannin and total phenol increased in the three species but only the amount of total tannin of Q. branti i was increased highly under the treatment of control: In addition, the survival percentage of Q. brantii was higher than the other species. Therefore, it seems that under the drought stress, Q. brantiimaintained a higher survival percentage through increasing the total tannin.