In order to study the various levels of irrigation and differentiation and maturity processes on seed vigor of two wheat cultivars, an experiment was carried out in Agricultural Research Center of Miandoab in 1387–1388. The experiment was conducted according to split-split plot based on completely randomized block design (CRBD), with four replications consisting four irrigation levels (70, 100, 130, and 160 bcA) and two wheat cultivars (Zarin and Shahryar) and ten harvesting times. Seed harvesting was carried out in seven days interval manner from 20 – 83 days after flowering. In this experiment, the weight of thousand seed, dry weight of seedling, germination rate, electrical conductivity of material seeped from the seeds, percentages of viable seeds and seed humidity was measured to determine seed vigor. The results of analyzed data indicated significant difference within irrigation levels in weight of thousand seeds and germination rate at %1 level. In addition, effects of harvesting times on all measured traits were significant in at %1 level. Interactions effects of irrigation levels ´ cultivar, on germination rate at %1 level and also on dry weight of seedlings and on electrical conductivity of material seeped from the seeds were observed on %5 levels. In the case of irrigation levels ´ harvesting times, only germination rate, was significant and in the case of cultivar ´ harvesting times, dry weight of seedling, weight of thousand seeds and germination rate were significant in %1 level. The effect of irrigation levels ´ cultivar ´ harvesting times, on electrical conductivity of material seeped from the seeds and germination rate was significant at %1 level. There was significant positive correlation between percentages of viable seeds, dry weight of seedling, germination rate and weight of thousand seeds, but significant negative correlation existed between electrical conductivity of material seeped from the seeds each measured traits. The results also indicated that cultivar Shahryar had more vigorous seeds and was superior to cultivar Zarin under stress conditions. Physiological maturity was observed between 55 – 62 days after flowering, which was suitable for seed humidity (14 – 15%) was harvested at the seventh stage (62 days after flowering), therefore it is suggested as a suitable time for harvesting.