Diatoma frequency in the Coastal waters of the Persian Gulf, between Tiab Creek to Bandar Lengeh was estimated from October 2003 to September 2004. In this monitoring five transects, (Tiab Creek, Fishing Jetty of Bandar Abbas, Nafti Jetty of Bandar Abbas, Bandar Khamir and Bandar Lengeh) were studied. In each transect, three stations and in each station, three depth (water surface, 2 and 5 meters) were sampled. 40 genera of Diatoma were identified and the following genera Nitzchia, Thallassionema and Pleurosigma were the most abundant. The highest and lowest diversity were observed in Auttum and Spring with 39 and 28 genera respectively. Average frequency of Diatoma were estimated 2004 cells per liter. The maximum and minimum frequency were obtaind in Summer and Spring with 4080 C/L and 1914 C/L respectively. Result showed that the greatest frequency were obtained in July and the lowest frequency were observed in January, the greatest and the lowest frequency were obtained in Fishing Jetty of Bandar Abbas and Khamir Port, respectively. Result showed that, by getting far from the coast, the frequency of Diatoms was increased in this studied area. (2 to 5 meters and surface layer). Studing the number of Diatoma in different depths showed that, Diatoma had higher frequency in depth of 5 meter in comparison with lower depth (0-2 meter). According to the result, No significant difference was observed in Diatoma frequency in different months, seasons and depths but significant diference was observed in different transcets (P<0.05).