VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ)
Year:2014 | Volume:8 | Issue:2 (30)|Papers Count:8

Ovarian cysts is one of the problems of dairy cattle with high production. The aim of this study was to evaluate the response to treatment of ovarian follicular cysts using calcium naloxone comparison with the conventional method of intramuscular GnRH injection. This study was conducted in third station dairy cattle farming center in Moghan on 54 cows' affected follicular ovarian cysts approximately 100 to 150 days after parturition. Following physical examination and diagnosis of the cystic cows, the animals were divided into three groups. The first group was the control group (5ml GnRH IM), the second group was the treatment group 1 (Nx via epidural injection 2 times with 3 day intervals) and the third group was the treatment group 2 (Ca-Nx via epidural injection 2 times with 3 day intervals and 5 ml of intramuscular GnRH simultaneously with the second Ca-Nx injection). To check the results, the animals was examined two weeks after the last injection. The results indicated that there was a significant difference in luteinization rate between the first and second groups with more luteinization rate in the first group. There was no significant difference between the first and third group in luteinization rate. Also, there was a significant difference in luteinization rate between the second and third group, with more luteinization rate observed in the third group. There was no significant difference between the fertility rate of the treatment and control groups.

It has been proved that Silymarin is effective in prevention and treatment of human liver diseases such as cirrhosis. Therefore, the objective of this study was to evaluate the preventive effects of Silymarin extract as a food additive on hepatotoxicity induced by carbon tetrachloride in broilers. Ninety broiler chickens were randomly assigned to 9 equal study groups as follow: negative control, positive control which were injected 2ml/kg BW (body weight) ccl4 dissolved  in olive oil (1:1) via IP  in the 24th and 26th days, olive oil control, treatment controls which were receiving 3, 6 and 10 mg/kg BW silymarin in 1ml olive oil respectively by gavage from the 21st up to 26th days  and treatment groups which received ccl4 and 3, 6 and 10 mg/kg BW silymarin extract respectively. Blood and liver samples were collected to assay ALT, AST and ALP activities as well as pathological changes. The Data were analyzed using ANOVA method by SPSS. The results revealed that carbon tetrachloride significantly increased ALT, AST and ALP activities in comparison with the negative control group (p<0.01). The 3, 6 and 10 mg/kg b.w. silymarin treatments significantly decreased the enzyme activities in comparison with the positive group (p<0.01). Histopathological findings confirmed the biochemical results. In conclusion, the results proved that silymarin treatment could be effective in the prevention of hepatotoxicity induced by carbon tetrachloride in Broilers.

In late June 2013, because of sudden and unjustified increase in mortality of apparent healthy fish in Hasanloo dam cage culture, random sampling was convicted from a live dying and dead fishes. Forty fishes (20 alive and 20 dead fishes) from two cage with the weight of 200-300 gr were sampled. Samples were sent to private factor laboratory ice pieces for evolution. Some physicochemical parameters of Hasanloo dam water including nitrite, nitrate, ammonium, soluble solid materials, temperature, dissolved oxygen and pH was measured. To investing the probability of bacterial, fungal and parasitic discover outbreaks sample were taken from the kidney, gills and skin of fish. The result of different investing revealed the presence of algal bloom, oxygen shortage, executive problems and fishing net fountain obstruction due to accumulation of planktons and suspended material. Because of increase in light intensity, nutritive substances level, warming of water temperature and static suability of water hydrological status, algal blooming had occurred in Hasanloo dam that not only destroyed the gills of fish but also created competition for soluble oxygen at night with some phytoplankton species causing hypoxia and death of salmon in the breeding farm. The result of general bacterial cutler was negative and mild secondary infection with S aprolegnia species was detected in PGYEA culture. The result of liver histopathology indicated the presence of hepatic ceroidosis because of non-standard diets with high levels of carbohydrates. Gill blade inflammation and hyperplasia was the observed. Due to high amounts of ionized ammonia among culture nets resolving from summer mortality syndrome.

The aim of this study was to compare the serum antibody titer of 8 types of commercial live infectious bronchitis vaccines in broiler chickens. Three hundred and twenty one-day-old male broiler chickens (308 Ross strain) were divided into 8 groups (each group comprised of 3 replicates) randomly. At 8 days of age, each treatment group received different types of live infectious bronchitis vaccine by eye-drop method. Twelve birds from each group were randomly chosen and blood sample was taken on the 22nd and 29th days of experimental period and ELISA test was performed on sera for detection of sample antibody titer. The mean of serum antibody titer against infectious bronchitis vaccines among treatment groups on the 22nd day of experimental period showed statistical significance between the group that had received HIPRAVIAR Colon/ H120 and the groups that had received Nobilis Ma5+ Clone30, H120 and Cevac Vitabron L (p<0.05) but no statistical significance was seen between the other treatment groups. The mean of serum antibody titer against infectious bronchitis vaccines among treatment groups on the 29th day of experimental period showed no statistical significance between the treatment groups. The comparison of serum antibody titer against infectious bronchitis vaccines in treatment groups between the 22nd and 29th day of experimental period showed statistical significance between the groups that had received Gallivax IB88, Nobilis Ma5+Clone30 and Cevac Vitabron L (p<0.05) but there were no statistical significance between the other treatment groups.

In this investigation, bacterial agents involved in pneumonia of slaughtered cross-bred cows in Tabriz abattoir were studied due to importance of bacterial pneumonia among Tabriz cattles. For doing so, we referred to Tabriz slaughter house every season and fifty healthy and fifty unhealthy lungs were sampled and transferred to the microbiology laboratory of Tabriz veterinary faculty, they were cultured then colonies were studied and finally the family and species of bacteria were recognized by using differential culture media. The separated bacteria from pneumonic lungs in four seasons involved 20 Streptococcus pneumoniae, 23 Escherichia coli, 18 Staphylococcus Aureus, 1 Staphylococcus epidermidis, 9 Corynebacterium pyogenes, 11 Pseudomonas aeruginosa, 48 Pasteurella haemolytica, 8 Erysipelothrix inscidiosa, 23 Rodococcus equi, 4 Nocardia farcinica, 2 Moraxella bovis, 1 Bordetella bronchiseptica, 2 Brucella bovis and 2 Haemophilus influenza. In four unhealthy lungs in fall, Pasteurella heamolytica with Bordetella bronchiseptica and from three lesioned lungs in fall, Brucella bovis with Haemophilus influenza and in three lesioned lungs in winter, Pasteurella heamolytica with Brodetella bronchiseptica were separated. The Bacteria isolated from healthy lungs consisted of 3 Staphylococcus epidermidis, 2 Pasteurella multocida, 6 Escherichia coli and 1 Nocardia farcinica. From 200 healthy lungs which were studied, 2 cases in spring, 4 cases in summer, 4 cases in fall and 2 cases in winter were isolated. It should be noted that, no bacteria was removed from primary culture of healthy and lesioned lungs in anaerobic conditions and mycoplasma culture.

Two hundred and forty one-day-old male broilers (Ross-308) in 6 treatments with 4 replicates and 10 birds in each replicate were used in this experiment. Dietary treatments consisted of: 1) diet with 5% vegetable oil (T1), 2) diet with 5% vegetable oil + 300mg/kg L-carnitine (T2), 3) diet with 5% animal fat (T3), 4) diet with 5% animal fat + 300mg/kg L-carnitine (T4), 4) diet whit mixed vegetable and animal fat (equal to 2.5%) (T5), 5) diet whit mixed vegetable and animal fat (equal to 2.5%) + 300mg/kg L-carnitine (T6). The diets with L-carnitine showed a significant effect on body weight in grower and whole growth period (p<0.05). Also adding L-carnitine in the diet increased carcass weight, leg and Breast meat and reduced abdominal fat (p<0.05). Adding L-carnitine in the diet had a significant effect on heart, liver, fabricious boursa, thymus and spleen weight (p<0.05). But adding L-carnitine in the diet had no significant effect on glucose, protein, albumin and globulins of blood (p>0.05). Also adding L-carnitine had a significant effect on HI Newcastle titer on the 32nd day (p<0.05) but it had no significant effect on HI Newcastle titer on the 42nd day (p>0.05).

The occurrence of abnormalities in different organs of the fetus and newborn of diabetic mothers has been proven today. Considering the irreversible damages of the disease in newborns’ reproductive system any action to reduce the abnormalities has an especial importance and necessity. In this experimental study, the protective effects of pumpkin powder on reducing testicular tissue damages of rats born from diabetic mothers has been studied. The pregnant rats were divided into 4 groups of 10 rats, as follows: 1) treatment group with pumpkin powder, 2) diabetic control group, 3) treatment group (diabetic animals treated with pumpkin powder) and 4) healthy control group. Experimental diabetes was induced in pregnant rats by intraperitoneal injection of 120 mg/kg b.w. alloxan monohydrate. The first and third groups received 2 g/kg b.w. pumpkin powder for 4 weeks via gavage. The histological and morphometric changes such as weight, seminiferous tubules diameter, spermatogonia, leydig and sertoli cell numbers were compared. Data was analyzed using the ANOVA and Tukey multiple comparisons test and p<0.05 was considered significant. Testicular. Testicular tissue histology of diabetic control group showed a marked decline in the numbers of seminiferous tubules compared with the healthy control and diabetic treatment group. In diabetic control group, scattered germ cells or spermatogonia was observed with a vacuolated cytoplasm suggesting cell degeneration, while in diabetic treatment group more germ cells without vacuolated cytoplasm were observed. There was a significant reduction of testicular weight in the diabetic control group compared with the diabetic treatment group. In diabetic control group, the diameter of germ cells, leydig and sertoli cells as well as the seminiferous tubules diameter had a significant reduction compared with the control group. In diabetic control group, pumpkin powder administration had increased the diameter of germ cells, leydig and sertoli cells as well as the seminiferous tubules compared with the diabetic treatment group. The results demonstrated that pumpkin powder had a protective effect against testicular tissue damage in fetuses born from alloxan induced diabetic mothers.

Newcastle disease is the most important viral disease that affected pigeons. The disease is characterized by sudden onset of anorexia and neurological symptoms in pigeon. Trichomonas gallinae causes trichomoniasis of pigeons in the upper gastrointestinal tract and the respiratory system. The symptoms of this disease include yellowish green fetid discharge from the mouth, diarrhea, emaciation, severe weakness and death. In the first 6 months of 1392, from a total of 32 suspicious cases from Tehran and its surrounding, swab samples of the mouth, pharynx and larynx of birds were prepared. The samples were studied for trichomonas infection. At necropsy, foci of white to cream color in the oral mucosa, pharynx, larynx and pharyngeal and tracheal mucous congestion associated with the presence of fetid fluid in the crop were observed. Also, general congestion of the carcass, urate deposition in the ureters, and the emptiness gastrointestinal tract was observed. For detection of Newcastle disease virus (NDV), samples of the trachea and spleen were collected and RT-PCR experiments were performed on the samples. Trichomonas was observed in the samples under the microscope. All of the 19 samples studied were considered positive to the presence of high virulence strain of the virus. Metronidazole and supportive therapies were used for treatment. Adherence to the principles of biosecurity, treatment or removal of trichomoniasis infected birds, and annual Newcastle disease vaccine are essential for the prevention of concurrent outbreak of these two diseases.