In this study algae Chlorella vulgaris Beijernick. after collection and purification were grown in the culture medium BG-11 containing aluminium (AlCl3) in 0 and 300mM concentrations, pH 7.1, 25±2oC and autotrophic and heterotrophic conditions for 21 days. In order to autotrophic conditions, light intensity of 1500Lux illumination for 20 hours and 4 hours with alternating light and darkness to create heterotrophic conditions, darkness with 0.05 gram of different sugars, glucose and sucrose was provided. In either case, survival and growth (based on spectrophotometry and cell counting), pH medium, fresh weight, total protein content, pigment content (chlorophyll a, chlorophyll b and b-carotene) and internal aluminium content in the logarithmic growth phase were studied. Therefore, aluminium significantly increased (P<0.05) growth, pH and fresh weight in autotrophic treatments containing 300mM aluminium was compared to other treatments. Chlorophyll a content of autotrophic cells of Chlorella vulgaris Beijernick. was nearly double that estimated in heterotrophic cells, while chlorophyll b content of autotrophic cells was nearly half the value recorded for heterotrophic cells. b-carotene content of heterotrophic cultures decreased by 31% compared to that value of autotrophic cells. A slight decrease in total protein content was observed under heterotrophic conditions. Survival during the 21-days cultures were maintained in darkness, but darkness conditions, the glucose tends was found. Sample in heterotrophic conditions (glucose), showed growth without lag phase which showed rapid acclimation of organisms in heterotrophic conditions. It seems as characteristics, single-celled green alga Chlorella vulgaris Beijernick. from different aspects, efficient and capable introduction to sample.