Purpose: This article aims to investigate the relationship between the personality characteristics of the librarians who are working in the libraries of Birjand Universities and their attitudes toward entrepreneurship in libraries (2014).Methodology: In this descriptive-analytic study which was of the correlation type, all the librarians who were working in the libraries of Birjand Universities in 2014 were included. The data collection included demographic forms (gender, age, level of education, field of study, work experience, and the service location) as well as a researcher-made questionnaire including 77 questions. The validity of the questionnaire was confirmed by five faculty members of librarians and its reported reliability was 0.94 using Chronbach's alpha. The data were analyzed using SPSS 15 software as well as the statistical tests of t-test, one-way variance analysis, Tokay follow-up range, Pearson correlation, and regression at the significance level of p≤0.05.Findings: Of 46 librarians under study, 15 cases were male (%32.6) and 31 cases were female (%67.4). The mean score of the librarians' attitudes regarding actions in the field of entrepreneurship in libraries was significantly higher in women compared to men. Besides, BA, MA, and doctoral librarians had a higher mean compared to those with diploma and associate degrees. Finally, the librarians of university of medical sciences outperformed Birjand, PayameNour, Azad, Farhangian, and Industrial Universities (p<0.05). The mean score of the librarians attitudes in the university of medical sciences regarding the reward system in library was significantly higher compared to the librarians attitudes in Birjand University (p=0.03). There was a significant and positive relationship between the prospective personal characteristic and entrepreneurship in the libraries accounting for %9 of entrepreneurial spirit in libraries (p=0.05).