Background and Aim: In developing countries, cervical cancer is the second most common cancer in women and in Iran the third is allocated between common cancers. Therefore, regular screening for the prevention of this disease is of great value. This study done، aimed to determine Pap smear screening behavior in women, according to the Trans-theoretical Model.Materials and Methods: In this cross-sectional study, selected 800 women referred to urban health centers Gonabad, with quota sampling method. The instrument was a researcher-made questionnaire including: demographic information, knowledge about cervical cancer and stages of change behavior pap smear screening of prochaska's model. The obtained data were analyzed by SPSS 16.Results: Mean age±SD of women was 36.76±9.25 years. The results indicate that 10.3, 8.4, 5.1, 13.1, 32.6 and 30.5 % of the women studied were in the stages of Pre-contemplation, Contemplation, preparation, Action, Maintenance, and Relapse, respectively .While 54.3 percent of subjects had experienced uterine infection , only 33.2% were aware of cervical cancer and how to recognize it. The statistical analyses show that there were significant difference between Pap smear screening behavior stage of change and age, marriage age, pregnancy age, pregnancy number, delivery number, child number and education, education and uterine infection background (P<0.005).Conclusion: Since over half of the women in this study did not perform Pap smear screening behavior (i.e., Pre-contemplation, Contemplation, preparation, and Relapse), it seems essential to devise appropriate educational programs based on stage of change model in order to make women aware of the significance of Pap smear screening behavior.