Hull-less barley is a cereal grain which can be considered as an energetically source of feed stuffs in poultry nutrition. For determination the nutritive value of this cereal, NSP as b-glucan, content of hull-less barley must be measured. The experiment was carried out with 20 hull-less barley’s promising line varieties that were produced in Iran from 2002-2004. Chemical compositions as dry matter, gross energy, crude protein, crude fat, ash, crude fiber, sugar and starch, were determined according to standard methods as AOAC 2000. NSP (b-glucan, as total, soluble and insoluble), content of hull-less barley were analyzed by using Megazyme assay kits. The results of experiment shows that there are significant differences (P<0.05) in chemical compositions (gross energy, crude protein, ash, starch, sugar) in hullless barley produced in 1381. Therefore, promising line 5 (Birjand) has the highest gross energy (4.35 kcal/g), crude protein (16.65%) crude fat (18.6), sugar (4.92%), starch (67.87), but amount of crude fiber has the lowest (1.4%) among the other lines. Also, total b-glucan content for promising line 2 (Darab) is the lowest and promising line 7 (Kerman) is the highest. The Soluble b-glucan content (%) is found in the range from 83-1.27 % and the highest belongs to promising line1 (Karj) and the lowest belongs to promising line5 (Birjand) and 6 (Yazd). The data obtained of hull-less barley varieties produced in 2004 shown that there are significant differences (P<0.05) for gross energy, crude protein, ash, sugar, starch and soluble and insoluble b-glucan. However, the highest grows energy, crude protein and starch belongs to promising line10 (Moghan) and line 5 (Birjand) respectively. Data show that there are significant differences (P<0.05) in soluble B-glucan content of hulless barley produced in 2004. In addition it was found that the highest amount of soluble b-glucan is for promising line 10 (Moghan). Comparing hull-less verities produced during 2002-2004 revealed that there are significant differences (P<0.05) in crude protein, ash, starch, sugar. Comparing products of these two years made it clear that the amount of crud protein and sugar in the first year are higher and the amount of crud fiber and ash are less than second year. However the content of soluble b-glucan in product of 1381 (0.89) is less than the product of 2004 (1.05).According to the results of the research the promising line 6 (Yazd) has proper nutrition value and the least NSP among other lines. So this paper proves promising lines 1 (Karaj), 2 (Darab), 3 (Isfehan), 5 (Birjand) 6 (Yazd) as the proper variety for different climates in Iran.