Background and Objective: Due to the increasing use of nano-materials, especially nano silver because of the anti-bacterial inevitable is used in various industries, it will entry into the ecosystems, so in this study, the toxic response of nano silver on goldfish was studied. Material and Methodology: To determine the toxicity of nano-silver, O. E. C. D standard method number 203 was used. Mortality recorded was daily basis over a period of 96 hours and after records, to determine the lethal toxicity in the 24, 48, 72 and 96 hours, probit software was used. Finally, the maximum allowable concentration and degree of toxicity was found. Findings: During the period of 24 to 96 hours with increasing concentrations of nano silver, mortality of fish was increased. The mortality rate at concentrations of 0, 0. 01, 0. 1, 0. 5, 1, 2. 5 and 5 mg after 24 hours were 0, 0, 0, 0, 4, 11 and 21 respectively, in concentrations of 0, 0. 01, 0. 1, 0. 5, 1, 2. 5 and 5 mg after 96 hours, mortality rates were 0, 0, 0, 10, 21, 21 and 21, respectively. Based on these results, the lethal concentration (LC50) of nano silver on goldfish in time 24, 48, 72 and 96 hours were obtained 2. 31 and 1. 48 and 0. 995 and 0. 53 ppm respectively. Discussion and Conclusion: In general, the results show that the toxicity of silver nano is very high during the finger period and its side effects should not be ignored with the increasing spread of this substance. It is also necessary to compare the results with silver ions when evaluating the toxic effects of nano-silver.