In order to evaluation the effect of zeolite application and selenium spraying on physiologic and agronomic traits of medicinal pumpkin (Cucurbita pepo L.) at different irrigation regimes, a experiment was conducted in factorial factorial based on randomized complete block design with three replications during 2010 in Takestan, Iran. In the where, the three levels of irrigation factor as the normal irrigation (control), withheld irrigation at the flowering stage and withheld irrigation at fruit formation stage, zeolite factor was at two levels of non application and use of 10 tons per hectare and selenium was sprayed at two concentration zero and 30 grams per liter per hectare. For this purpose traits such as chlorophyll index, RWC, fruit weight, seed number, seed length and width, 100 seed weight, seed yield and oil percent were evaluated. The results showed that water deficit stress reduced all traits were evaluated. Zeolite application of 10 tons per hectare in water deficit stress conditions, improved traits such as chlorophyll index, RWC, seed number, 100 seed weight, seed yield and oil percentage but its application in normal irrigation conditions had not significant effect on mentioned traits and only increased the number of seeds per fruit. Selenium spraying under total irrigation levels, had a positive effect on evaluated traits. In normal irrigation, Selenium spraying increased RWC, fruit weight, number of seeds per fruit and fruit yield and in stress conditions also had positive effect on RWC and seed yield.