Determination of blood parameters, blood tissue and Hematological analysis in fish can be good indicators for the detection and determination of health and or the infectious diseases. The purpose of this research, Determination of hematological parameters of cultured Acipenser baerii. In three different ages of groups, with the weighted average, Fry fish (21.5±3.9), Juvenile fishes (198±19.7) and Spawners (female, 668.6±73.9 and male 533.8±160.8) in a period of six months. For this reason, 30 numbers of cultured Acipenser baerii were used (10 fry fishes, 10 Juveniles fishes, 5 female spawners fish and 5 male spawners fish). The results indicated that compared hematological parameters average, all parameters except for RBC, MCV, MCH, MCHC significant differences were observed during different ages (p<0.05). But in terms of Sex statistically significant difference was not seen (p>0.05). By investigating the present results, it can be stated that age, and sex is the most effective in the change of blood parameters.