Background: We aimed to explain the roadmap to supremacy in the country's medical universities. Expanding this path in all higher education institutes would improve supremacy. Methods: In this project, local competitive advantages and the strengths of medical universities were initially identified. Three methods were used for measuring and quantifying, as follows: determining the index based on total citations of the top ten researchers in each field and the ratio of these people to the top ten in the country, SciVal analysis, evaluation and internal evaluation of universities and institutions were used. Results: The data collected from the previous two stages during 2020-2021 led us to potential areas of differentiation. Strategic placement took place with an emphasis on the division of national duties and special missions, and we paid attention to the articulated diferentiated. All competitive advantages were classified into four levels (internal, weak, strong, and exclusive competitive advantage) based on characteristics such as infrastructure, capital and resources, access, reputation, staff, processes, and various functions of universities. The way of moving between the start point, and the destination was also done by formulating strategies. Conclusion: In formulating a strategic plan for achieving supremacy, a strategic timeline, a strategic roadmap, and a strategic plan were developed. Supremacy is a high goal for universities. However, universities must also consider other goals, such as: creating knowledge-based economic value addition, interdisciplinary development, social accountability, and the institutionalization of different levels of academic hubs in universities. In this path, identifying and encouraging talented individuals and making full use of their scientific capacities is also very important.