The purpose of this study is to investigate the factors influencing on ambidexterity of employee behaviors and its consequences with a multi-level approach. This research is empirical according to purpose and descriptive-survey based on data gathering method. The statistical population of this research is 17000 employees of 20 petrochemical companies located in Mahshahr Special Economic Zone. In this study, Chen, Gowley and Eden (2001), Mam et al (2009), Jansen (2000), Patel and colleagues (2013) questionnaires are used to measure self-efficacy, behavioral ambidexterity, innovative behavior, and high performance work system, respectively. Results of the study showed that self-efficacy has a positive and significant effect on ambidexterity at individual level. Also, Individual ambidexterity has shown a positive and significant effect on the innovative behavior of employees. The mediating role of behavioral ambidexterity between self-efficacy and innovative behavior of employees was confirmed. At the organizational level, the contextual effect of high performance work system on behavioral ambidexterity, self-efficacy and innovative behavior of employees was confirmed. It was also found that a high performance work system moderates the effect of self-efficacy on behavioral ambidexterity and also moderates the effect of behavioral ambidexterity on innovative behavior of employees. This study showed that personality traits along with the of the high performance work system variable can enhance the behavioral ambidexterity of employees in their work and thus lead to improve their innovative behavior.