Fifteen populations of the sweetpotato whitefly, Bemisia tabaci Gennadius (Homoptera: Aleyrodidae) from Ahwas, Esfahan, Ilam, Bushehr, Tabriz, Tehran, Ramsar, Rasht, Semnan, Shiraz, Kashmar, Kerman, Gorgan, Macko, and Yazd were morphologically studied using Scanning Electron Microscope. The morphological characteristics of fourth nymphal instars were studied considering the presence or absence of anterior submarginal setae, length and width of anterior and posterior wax margins, length of dorsal setae, presence or absence of posterior submarginal setae, length of caudal setae, and width of thoracic tracheal folds. The studied characteristics on adult males were the number and diameter of dorsal (discoidal) disks. Except for the diameter of dorsal disks, significant differences were observed between populations for other morphological characteristics. Morphological comparisons indicated that, it is possible to identify the different biotypes of B. tabaci through morphological characteristics. Therefore, biotype B (Bemisia argentifolii Bellows & Perring) exists in the Southern regions of Iran including, Ahwaz, Esfahan, Shiraz, Kerman, Yazd, and Bushehr as the dominant biotype, but this biotype does not present in other regions. The studied differential morphological features of various populations was resented in tables and microscopic photographs.