The unexpected increase in the price of bitumen and production capacity of cement have provided the tendency to replace Asphalt pavements with concrete. Considering the high cost of material supply and the implementation of pavement and despite the limitations of construction credits, there must be serious thought and comprehensive planning to improve the quality and increase the durability of pavement roads, which will consequently reduce maintenance costs. One of the most important breakdown of concrete pavement is cracking. Also one of the most widely used building materials, concrete has a brittle behavior. Adding fibers to concrete affect its tensile strength ductility behavior, flexural strength, elastic modulus, impact resistance as well as some mechanical properties. Fiber-reinforced concrete is a type of concrete that is mixed with fiber. Various types of fibers are used to produce fiber-reinforced concrete, which include glass, polymer, carbon and steel. In the present research, macro-synthetic polymer fibers with twisted shape were used. According to the past studies Some of the consequences of applying macro-synthetic fibers in concrete include reduced shrinkage of fresh and hardened concrete, increased ductility, increased strength against fatigue stresses, increased durability and lifetime of concrete, improved concrete mechanical properties (tensile strength, flexural strength, etc. ), control of secondary/thermal cracks of concrete, preventing the in-depth propagation of cracks, post-cracking chargeability and reduced permeability against chloride and sulfate ions. In most of the studies, the concrete sample's thickness is increased along with the increase in the beam's length; however, in the present work, only thickness of the beam samples with and without fibers was changed and other dimensions of the samples were kept constant in order to investigate merely the effect of increased thickness. Accordingly, effect of the size of macro-synthetic fiber-reinforced concrete sample at different thicknesses was assessed via fatigue life variations. The intertwisted fibers were added to the concrete mixture by 0. 4 vol. %. Then, from each sample, three specimens were made. The obtained results were averaged and, then, recorded in the relevant tables. The cases considered as the research objectives are Effect of sample size on fatigue life of concrete samples and Effect of adding macro-synthetic fibers on fatigue life. In order to evaluate the flexural fatigue life of concrete beams, the concrete mixture was designed based on ACI 211 Standard. All the concrete samples were made with the same mix design and 0 and 0. 4 volume percentages twisted fibers were used for the fibrous mixtures. Hence, an experimental study was conducted to provide an experimental model of the flexural fatigue life of macro-synthetic fiber-reinforced concrete by constructing concrete beams with three different thicknesses of 80, 100 and 150 cm. S-N models (Stress level – Number of loading) and H-N (Thickness-Number of loading) models were presented. The obtained results showed that increasing the thickness of the concrete samples and adding fibers to the concrete mixture would lead to the increased fatigue life. Also, the addition of fibers to concrete specimens showed the thickness of the specimen decreased for the stress level of 0. 7, 0. 8 and 0. 9 by 8. 24-12. 45%, 5. 5-22. 5% and 10-22. 95% Respectively.