To evaluate the distinctive morphological characteristics of cotton cultivars, and the correlation among different traits of cotton species, current research was conducted on Sahel, Golestan, Sepid, M-13, SKT-133, Latif, Termez 14, Barbadense and Doctor Omumi cotton cultivars from 2 different species of Gossypium hirsutum and G. barbadense in randomized complete block design (RCBD) during 2015 year in Hashemabad Cotton Research Station, Gorgan, Iran. Studied traits were included of plant height, number and length of sympodia and monopodia branches, the nodes numbers, bolls number and weight, seed weight, yield and lint percentage. According to the results, for all of the studied traits, significant statistical difference (p<0.01) were observed. This matter represents clear and distinct differences among evaluated cultivars. Characteristics of monopodia and sympodia branches did not follow a particular trend between the cultivars of two species and these traits were not considered depending on the plant species. Furthermore, the characteristics of plant height, number of bolls, boll weight, seed weight and lint percentage can be attributed to species type, so that Doctor Omumi cultivar from G. barbadense species and SKT-133 from G. hirsutum species produced the highest and lowest number of bolls, respectively. Boll weight was varied from 3.25 gr in Barbadense cultivar to 5.29 gr in Sahel cultivar. The highest lint percentage (42.2 %) was related to Golestan cultivar and the Barbadense cultivar from G. barbadense species had the lowest amount of lint percentage. Seed cotton yield had a significant positive correlation with boll weight and first harvesting. In contrast, there was a negative correlation between the number of bolls and lint percentage. According to the group comparisons, the G.hirsutum considering the traits: total yarn 10.9 %, length of the highest monopodia branch 27.48 %, first cut yield 753.15 Kg/ha, total yield 719.55 Kg/ha and the and boll weight 1.39 gr in comparison with G.barbadense was superior in a way that being high these traits compared to other agricultural traits had the most effect on total yield of the crop. Accordingly, knowing about the relations among the traits and their effect on the total yield can be helpful in increasing the production of this important plant.