IRANIAN SOUTH MEDICAL JOURNAL (ISMJ)
Year:2012 | Volume:15 | Issue:2|Papers Count:11

Background: Two Dimensional Electrophoresis (2DE) is the most commonly and useful separation technique in proteomics. Each proteome snapshot becomes a protein profile. By means of this technique, several proteins are studied simultaneously.Methods: In this study, by use of (2DE) method, the differences of two profiles of Buthidae endemic scorpions, A.Crassicauda known as "black scorpion" and "O.doriae" yellow scorpion, were investigated.Results: For A.Crassicauda scorpion there were about 20 spots (peptides) in 6.2-8.2 pH ranges and molecular weight was less than 3 to 14 kDa and O.doriae scorpion 30 peptides, in 6.3-8.5 pH ranges, 1 to 45 kDa that fractionated and identified.Conclusion: By this method, the field of bioinformative data bank from Iranian endemic scorpions' venom could be prepared. By making change of any effective factors on scorpion venom, considerable results due to influence of the factor on determining kind of venom can be achieved and studied.

Background: Coagulase Negative staphylococci (CoNS) are nosocomial pathogens. The main objective of the study was to compare the performance of disk diffusion, E-test and agar screening methods with Real-time PCR technique for detection of methicillin resistance in coagulase negative staphylococci (MRCoNS), using Taqmanâ Real-time PCR for mecA DV WKH3 JROG VWDQ GDUG' FRP SDULVRQ DVVD. Methods: 88 coagulase negative Staphylococcus isolates were identified out of 284 Staphylococcus isolates collected from Hajar and Kashani hospitals-shahrekod, Iran. Methicillin resistance strains were identified by several methods: Disk diffusion, Agar screening, E-test and Real-time PCR. The results of the tested methods ZHUH FRP were compared with those of the Real-time PCR by Chi square or Fisher's exact tests.Results: Of the 88 coagulase negative Staphylococcus isolates tested, 46 isolates (52.3%) were mecApositive and 42 isolates (47.7%) were mecA-negative. The results of all the tested methods had a statistically significant agreement with those of Real-time PCR. The E-test was 100% sensitive and specific for mecA presence. The sensitivity and specificity of oxacillin agar screen (6.0 mg/ml) method were 96% and 98%, respectively and the sensitivity and specificity of oxacillin Disk diffusion method were 91% and 90%, respectively.Conclusion: In the present study, E-test is proposed as the best phenotypic method. In case economic issue matters, the oxacillin agar screening method (6.0 mg/ml), which is suitable for the detection of MRCoNS due to its accuracy and low cost, is recommended.

Background: Obesity-related disorders characterized by insulin resistance such as metabolic syndrome, hypertension, coronary artery disease and type 2 diabetes are associated with decreased adiponectin levels. The purpose of the present study was to determine association of lipid profile and obesity markers with serum adiponectin in type 2 diabetes adult males.Methods: For this purpose, forty one adult males with type 2 diabetes were enrolled in this study randomly. Blood samples were collected after a 10-12 hour overnight fasting for measuring serum glucose and lipid profile and adiponectin. The anthropometrical indices were measured too. A multivariate linear regression analysis used to predict relation between variables.Results: The data analysis showed that circulatory adiponectin had significant negative association with glucose, triglyceride, total cholesterol, visceral fat and cholesterol/HDL ratio (P£0.05). There were no significant correlation between adiponectin level and HDL and LDL.Conclusion: Our findings support negative relationship between serum adiponectin and cardiovascular risk factors in diabetic patients.

Background: Inactivity is a leading contributor to chronic health problems. Physical activity (PA) is an important element in maintaining the health and functional ability in the population and has favorable effects on lipid profile in adults. Here, we examined the effects of pedometer-based PA (step/day) in healthy middle age men.Methods: Height, weight, body mass index (BMI), and daily oxygen consumption (VO2peak) were measured in 27 active and sedentary healthy middle aged men (40-65 yr). Subjects wore a pedometer throughout the day for three consecutive weeks, and average steps per day (physical activity volume) were measured. Fasting serum concentrations of total cholesterol (TC), triglyceride (TG), high density lipoproteincholesterol (HDL-C), and low density lipoprotein-cholesterol (LDL-C) were measured.Results: Mean of Daily steps in the Active group (AG) (12632±3957 steps/day) was significantly higher than sedentary group (SG) (5347±1631 step/day) (p<0.001). Also There was a significant difference in daily oxygen consumption in the 2 groups, with higher values found in the AG (p<0.001). TG and TC concentrations in the AG were significantly lower than SG (p<0.05). TC/HDL-C and LDL-C/HDL-C ratios in the AG were significantly lower than SG (p<0.05), whereas, HDL-C and LDL-C concentrations were not different in the two groups (p>0.05). A significant correlation was found between average steps per day and TG (-0.424, P=0.027), TC (-0.389, P=0.045), TC/HDL-C (-0.469, P=0.014), HDL-C/LDL-C (-0.390, P=0.044), and Daily Oxygen Consumption (-0.853, P=0.000).Conclusion: It seems that, healthy middle-aged men, who have more ambulatory activity (average of 12, 500 step/day), have significantly better blood lipids' profile.

Background: Given the longevity achievable with current prophylactic and therapeutic strategies for persons with HIV infection, quality of life (QOL) has emerged as a significant medical outcome measure, and its enhancement has an important goal. This review highlights the relevance and complexity of physical, psychological, and social factors as determinants of health-related quality of life in HIV-infected person in Bushehr province.Methods: This cross-sectional study was conducted using a convenience sampling method on 54 patients living with HIV or suffering from AIDS on 2009. The method of data collection was summarized questionnaire of World Health Organization (WHO-QOL-Brief). The main measured outcome in this study was quality of life and some related demographic and clinical variables.Results: The majority of the patients were males (46.54) and unemployed (37).25 participants were married. The mean±SD age of the patients was 38.1±7.47 years. Employment, marital status, level of education, and clinical stage of the disease, had a significant effect on the quality of life of the patients. In multivariate analysis, the most important predictor of the quality of life was job.Conclusion: The most important factors, association with decreased quality of life of the Patients in this study, were being separated or divorced, unemployment and being at severe stage of the disease. The relationship of these measures to treatment outcomes can and should be examined in clinical settings in resource-limited countries.

Background: Appropriate location selection for health Services unites is a Strategic decision which is based on gathering information and also exact analysis of related alternatives. It's clear that result of this decision is really important and will led to long time improvement in all dimensions including economic, social, and etc. Otherwise, the most studies of location have done in background manufacturing rather than service area and there are just few articles which have focused in field of healthcare. This study attempted to solve this stock of researches.Methods: By using an almost comprehensive literatures review, objective and subjective criteria are identified. After finalizing criteria's, Are used from decision-makers' assessments for collection data. Fuzzy Analytical Network Process is used for analyzing the data.Results: The finding indicates that the criteria's of proposed model are high validity and reliability for appropriate location selection of Health Centers.Conclusion: The analyzing of data indicates that lamtar locate is selected appropriate location.

Background: Life style Changes during Ramadan month could possibly affect sleep-related behaviors such as total daily sleep time, sleep and wake up time and brain waves. In addition, Spirituality and religiosity have a marvelous influence on mental health and effective solutions against stress are being religious and believe in God. This review discusses the results of all related studies about possible effects of Ramadan fasting on various aspects of sleep pattern and mental health.Methods: keywords such as 'Ramadan', 'Ramadan Fasting', 'Islamic Fasting', 'Fasting in Ramadan' and Fasting along Sleep, Chronotype, Sleep Latency, REM, NREM, Brain Wave, Psychology, Mental health, Religion, Mood, Depression, Social interaction, Depressive illness, Psychomotor performances, Bipolar disorders, Accident, Mania, Anxiety and Stress were searched via PubMed database, Scientific Information Datebas (SID) and also some local journals, hence, 103 related articles from 1972 until 2010 were studied.Results: The results of studies about the effects of Ramadan fasting on sleep pattern is not similar and these differences could be due to cultural and life style discrepancy in several countries. Fasting during Ramadan could lead to delay in sleep-wake cycle, decrease in deep sleep and lack of awareness during the day.Conclusion: There are various reasons such as dietary pattern, hormonal changes and also stress which could alter the quantity and quality of sleep during Ramadan. Also, according to the available information, there is a relationship between fasting and mental health.

Uterine artery pseudo aneurysm is a rare cause of late postpartum hemorrhage following Cesarean or uncomplicated vaginal delivery, gynecologic surgery such as dilatation and curettage, hysterectomy and myomectomy. In this article a case of uterine artery pseudo aneurysm is introduced. Patient is a 27-yearold female, Gravida 2, Para 2 who presented to the emergency department with episode of heavy vaginal bleeding after a cesarean section in 42 days ago. In clinical examination there was no pathological sign except paleness and agitation. In gynecological examination there was vaginal bleeding more than normal menstruation bleeding, cervix was dilated 1 centimeter and uterus was about 14 weeks of pregnancy. A diagnosis of pseudo aneurysm of the uterine artery was diagnosed by Doppler ultrasonography and Total abdominal hysterectomy was performed. The aim of presenting this case is emphasis on considering uterine artery pseudo aneurysm as one of the causes of late postpartum hemorrhage. In these situations Doppler ultrasonography is essential for diagnosis. Patient situation and hospital equipments determine treatment methods.

Aging is associated with reduced bone mass in men and women. Women loose about 35% of their cortical bone and 50% of trabecular bone since middle of 2nd decade of life. Subsequently, bone mass reduction increases fracture in women. Femoral bone fracture is associated with chronic low back pain, scoliosis, limited range of movement and more hospital admission. Prevalence of osteoporosis is increasing. It has been estimated that direct therapeutic costs and indirect costs due to reduced economical productivity of osteoporotic patients will increase 2 or 3 times. Moreover, it is also estimated that more than half of femoral bone fractures' of the world will occur in Asia. Therefore, there is need to further explore different aspects of osteoporosis locally. This book reviews physiology, diagnostic methods and different treatments of osteoporosis. It also discusses pathophysiological and diagnostic complexities of osteoporosis. Osteoporosis essentials are presented in combination with its new therapeutic findings. Therefore, the book could be used as a comprehensive tool for improving and updating current knowledge of readers about osteoporosis.