Journal of Shahid Sadoughi University of Medical Sciences
Year:2018 | Volume:26 | Issue:5 |Papers Count:10

Introduction: Ankle sprain is one of the most common injuries among the athletes. According to some research, balance deficit and ankle instability are reported in those with chronic ankle instability. The aim of this study was studying the effect of 6 weeks' new balance training in the land and water on balance, instability index and pain in the athletes with chronic ankle instability. Methods: In this semi-experimental study, 30 athletes with chronic ankle instability were selected with purposeful sampling and randomly were placed in two groups (aquatic and land therapy). Pain, instability, the static and semi-static balance were measured pre and post training. The intervention included the sixweek modern balance training in the water and land. To analyze the data, SPSS (22) software was used. to compare the results of the pre-test and post-test, paired sample t-test and to compare the means between two groups, the independent sample t-test was used. The significance level was 0. 05. Results: The results showed that both experimental groups had a significant difference in the instability index (P=0. 001), static (P=0. 001) and semi-dynamic (P=0. 002) balance compared to the pre-intervention stage, but there was no significant difference in pain variable (P=0. 38). However, comparison of the means of the studied variables between the two groups did not show a significant difference in the instability index (P=0. 1), static (P=0. 8, P=0. 6), semi-dynamic (P=0. 9, P=0. 5) and pain (P=0. 8). Conclusion: Many clinicians propose water-based exercises in the ankle injured athletes, which are costly in addition to time-consuming. The results of this study suggest that the patients with chronic ankle instability can rehabilitate by use of available tools, even at home.

Introduction: In the recent decades, the prevalence of Klebsiella pneumoniae beta-lactamase producing strains has a significant role in nosocomial infections. The aim of this study was to determine the prevalence of different types of CTX-M enzymes among clinical isolates of K. pneumoniae. Methods: In this descriptive cross-sectional study, during six months, K. pneumoniae strains were isolated from laboratory ward of Babol educational hospitals and referred to microbiology laboratory of Babol University of Sciences for final confirmation. Thereafter, antibiogram pattern analysis and the frequency of CTX-M1 and CTX-M15 genes were evaluated. Finally, the results were analyzed by SPSS version 24 software. Results: During six months of this study in 2017, 65 K. pneumoniae and also other bacterial strains such as Escherichia coli, Pseudomonas aeruginosa, and Acinetobacter baumannii were collected from laboratory ward of Shahid Beheshti and Ayatollah Rouhani Hospitals. By disc diffusion method, the highest resistance belonged to Amoxicillin (91%), Cefoxitin (45%), Piperacillin (45%) and the lowest belonged to Amikacin (24%) and Imipenem (0%). On the other hand, the percentage of CTX-M1 and CTX-M15 genes were also found to be 62% and 69%, respectively. Conclusion: Regarding to the high prevalence of strains containing broad-spectrum beta-lactamase enzymes and due to the antibiotic resistance among K. pneumoniae strains isolated from clinical specimens and also by considering this issue that the current study was the first research for evaluating the frequency of these genes in Babol educational hospitals therefore, it is necessary to adopt appropriate drug regimens to reduce antibiotic resistances.

Introduction: Hamstring and patellar ligaments are the most common ligaments that used in arthroscopic anterior cruciate ligament reconstruction (ACLR). Preoperative knowledge about the diameter of the graft can be so useful for the surgeons. In the current study, the efficacy of measuring the graft diameter using MR imaging was investigated. Methods: In the present study 50 patients with ACL tearing who referred to Yazd Shahid Sadoughi Hospital during the first 6months of year underwent ACLR. Before the operation, the diameter of the hamstring graft was measured on MR images. Further, the true diameter was measured intraoperatively. Two measurements were compared and the presence of correlation between the graft diameter and BMI, weight and age was investigated. The data were analyzed by Spss software 19 and t-test. Results: The average diameter of graft was 6. 2± 0. 9 mm on MR images and 7. 4± 0. 8 mm intraoperatively. The difference was not statistically significant (p=0. 315). Furthermore, there was a positive significant correlation between two measurements (r=0. 374, p=0. 025). There was, also, a positive significant correlation between the graft diameter and weight (r=0. 264, p<0. 001). Conclusion: The results of the present study show that the MR images can be used as a reliable tool for estimating the hamstring graft diameter before ACLR.

Introdution: Regarding to the development the problems of systemic toxicity and drug resistance in cancer chemotherapy, the continuing discovery of new bioactive compounds and anticancer agents is very necessary. Therefore, in this study, we checked the anticancer activity of native Pseudomonas sp. UW4 metabolite to find new compound. Methods: This experimental study was performed in Shahrekord Islamic Azad University from April 2015 to August 2015. First SK-BR-3 human breast cancer cells with different concentrations (5, 10 and 20 mg/ml) were treated with produced metabolite by native Pseudomonas sp. UW4 for 24 and 48 hours. Cell viability was assessed by MTS assay. Then, p53 expression was detected by RT-Real Time PCR. For statistical analysis, SPSS16 software and one way ANOVA test were used. Results: Treatment with produced metabolite by the native Pseudomonas sp. UW4 showed the decreases the viability of cells in a time and dose dependent manner, the most effective concentration of this substance was 20 mg/ml and 48 h after treatment. Also, an increase in p53 gene expression, significantly in 10 and 20 mg/ml after 24 h and 5, 10 and 20 mg/ml after 48h was observed (P<0. 05). Conclusion: Produced metabolite by native Pseudomonas sp. UW4 could be used for treatment of SK-BR-3 breast cancer.

Introdution: The aim of this study was to investigate the mediating role of cognitive emotion regulation strategies on the relationship between attachment styles and quality of life in the patients with multiple sclerosis in Tehran. Methods: The research method was descriptive-correlational. The statistical population of the study was all the patients with multiple sclerosis referred to Sina Hospital in Tehran in 1396. By using the sample size calculations in structural equations and available sampling method, 354 participants were selected as a sample size. In order to measure the variables, the cognitive emotion regulation strategies Questionnaire (Garnefski et al., 2001) (), Hazan and Shaver Attachment Styles (1990) and World Health Organization Quality of Life (1997) were used. The proposed model was evaluated by structural equation modeling using SPSS21 and Smart-Pls 2 and Amos-22 softwares. The technical characteristics of the questionnaire, including reliability, convergent validity and divergent validity were investigated. Moreover, path coefficients and software significance coefficients were used to examine the research hypotheses. Results: The results of the present studyshowed that cognitive emotion regulation strategies have a mediating role in attachment styles and quality of life. Conclusion: Therefore, the results of the study can lead to a better understanding to promote mental health after the onset of multiple sclerosis.

Introdution: The statistics of infertility among couples is about 15%. 60% of infertility cases are related to male factors, and the share of infertility due to genital tract infection is 20%. In bacteriospermia, the presence of bacteria and leukocytes produce active forms of oxygen and damage to sperm cells. In the present study, determination of frequency and diversity of bacteria involved in urinary tract infection, determination of malondialdehyde in sperm and seminal plasma as an index of oxidative damage and its relation with microbial contamination and effect of oxidative stress and microbial contamination on the quality of semen was evaluated in infertile men. Methods: This trans-sectional study performed on 53 patients referred to the Infertility Treatment Center of Jahad Daneshgahi. After sampling, semen parameters according to WHO (1999) protocol and bacterial contamination by culture and differential tests and also malondialdehyde concentration in sperms and seminal plasma via fluorimetric method were determined and results analyzed by ANOVA and T test in SPSS16 software. Results: Based on the results, 92% of the patients had bacterial contamination, including Staphylococcus aureus (65%), Staphylococcus epidermidis (24. 5%), Proteus mirabilis (2. 4%), Klebsiella (4. 8%) and Enterococcus (4. 8%). Bacteriospermia had not only a significant effect on the quality of semen, especially sperm motility (p<0. 01), but significantly increased (p<0. 01) the malondialdehyde concentration in patients' cells and seminal plasma. Conclusion: Infection affects semen quality and decreases the chance of a successful ART. Malondialdehyde can be used as a quick and accurate indicator for evaluating bacterial contamination in infertile men.

Introduction: Ability of tooth shade segregation by A dentists is very important. Assessment of tooth shade selection by dentists is one of the most basic investigations. In this survey, dentistry students’ ability of tooth shade selection and their progress by education was evaluated. Methods: This study was done on 59 Dentistry Students of Shahid Sadoughi Dental School of Yazd. 32 of the students were studying in the 9th semester and 27 were in the 11th semester. For assessment of tooth shade selection ability, dentistry students were asked to adapt 4 composite samples to Vita Classic shade guide. Results: The ability of complete shade matching was seen only in %5. 1 and ability of 3, 2, and 1 or less color segregation sequence was seen in% 35. 5, %33. 9 and %25. 5 respectively. The ability of shade selection was better among the students of the 11th semester than the students of the 9th semester (P value=0. 004). Sex has no effect on ability of shade selection by students (for 9th semester P value = 0. 198 and for 11thterm, P value = 0. 495). There was no significant difference between using glasses or lenses compared to students direct vision In two semesters. Conclusion: In this study, seen education improved the ability of shade selection by students but sex or wearing glasses or lenses was not effective.

Introdution: Bisphenol A (BPA) disturbs the morphology, viability and differentiation of rat bone marrow mesenchymal stem cells (rMSCs) to osteoblast via the production of free radicals. Vitamin C (vit-C) is a potent antioxidant and protects cells against oxidative stress. The aim of this study was to investigate the effect of co-treatment of BPA and vitamin C as an antioxidant on the viability and osteogenic differentiation of rMSCs. Methods: In this experimental study, rMSCs were divided into 4 groups; control, BPA (200nM), BPA (200nM) + vit-C (300μ M) as well as vit-C (300μ M) and treated for 21 days in the osteogenic media. Then, cell viability, osteogenic differentiation, morphological changes and DNA breakage in different groups of cells were evaluated. Data were analyzed using one-way ANOVA and Tukey‘ s test and the means were considered significantly different at P<0. 05. Results: A significant reduction in the cell viability, bone matrix mineralization, alkalin phosphatase activity and intracellular calcium concentration (P<0. 001) as well as a considerable increase in DNA breakage was seen in the BPA group compared to the control. The above parameters were compensated in the BPA + Vit-C group to the control level. Conclusion: The results of this investigation showed that Vit-C can compensate the adverse effects of BPA on the viability and osteogenic differentiation of rMSCs.