Survey the effect of produced metabolites by native Pseudomonas sp. UW4 on p53 gene expression in SK-BR-3 breast cancer cell line

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Pourhavashemi Setareh; BAMZADEH ZAHRA; ROUHI LEILA; ZIA JAHROMI NOOSHA

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Journal: Journal of Shahid Sadoughi University of Medical Sciences Year:2018 | Volume:26 | Issue:5 Page(s): 399-409

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Information Journal Paper

Title

Survey the effect of produced metabolites by native Pseudomonas sp. UW4 on p53 gene expression in SK-BR-3 breast cancer cell line

Author(s)

Pourhavashemi Setareh | BAMZADEH ZAHRA | ROUHI LEILA | ZIA JAHROMI NOOSHA | Issue Writer Certificate

Keywords

Pseudomonas sp.UW4Q1

MetaboliteQ2

Breast cancer SK-BR-3Q1

Cell viabilityQ1

p53 geneQ2

RT-Real Time PCRQ1

Abstract

Introdution: Regarding to the development the problems of systemic toxicity and drug resistance in cancer chemotherapy, the continuing discovery of new bioactive compounds and anticancer agents is very necessary. Therefore, in this study, we checked the anticancer activity of native Pseudomonas sp. UW4 Metabolite to find new compound. Methods: This experimental study was performed in Shahrekord Islamic Azad University from April 2015 to August 2015. First SK-BR-3 human breast cancer cells with different concentrations (5, 10 and 20 mg/ml) were treated with produced Metabolite by native Pseudomonas sp. UW4 for 24 and 48 hours. Cell viability was assessed by MTS assay. Then, p53 expression was detected by RT-Real Time PCR. For statistical analysis, SPSS16 software and one way ANOVA test were used. Results: Treatment with produced Metabolite by the native Pseudomonas sp. UW4 showed the decreases the viability of cells in a time and dose dependent manner, the most effective concentration of this substance was 20 mg/ml and 48 h after treatment. Also, an increase in p53 gene expression, significantly in 10 and 20 mg/ml after 24 h and 5, 10 and 20 mg/ml after 48h was observed (P<0. 05). Conclusion: Produced Metabolite by native Pseudomonas sp. UW4 could be used for treatment of SK-BR-3 breast cancer.

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APA: Copy

Pourhavashemi, Setareh, BAMZADEH, ZAHRA, ROUHI, LEILA, & ZIA JAHROMI, NOOSHA. (2018). Survey the effect of produced metabolites by native Pseudomonas sp. UW4 on p53 gene expression in SK-BR-3 breast cancer cell line. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 26(5 ), 399-409. SID. https://sid.ir/paper/36630/en

Vancouver: Copy

Pourhavashemi Setareh, BAMZADEH ZAHRA, ROUHI LEILA, ZIA JAHROMI NOOSHA. Survey the effect of produced metabolites by native Pseudomonas sp. UW4 on p53 gene expression in SK-BR-3 breast cancer cell line. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2018;26(5 ):399-409. Available from: https://sid.ir/paper/36630/en

IEEE: Copy

Setareh Pourhavashemi, ZAHRA BAMZADEH, LEILA ROUHI, and NOOSHA ZIA JAHROMI, “Survey the effect of produced metabolites by native Pseudomonas sp. UW4 on p53 gene expression in SK-BR-3 breast cancer cell line,” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 26, no. 5 , pp. 399–409, 2018, [Online]. Available: https://sid.ir/paper/36630/en

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