Increasing oxidative stress is a major factor in metabolic syndrome associated with obesity and may play a major role in the pathophysiology of various diseases such as cardiovascular diseases. The aim of this study was to investigate the effect of 8 weeks of rope training with portulaca supplementation on antioxidant biological indices (SOD, TAC, GPX) and lipid peroxidation index (MDA) in non-active girls. In this quasi-experimental study, 40 overweight and obese girls (mean BMI=27. 05 ± 0. 72, age range=20-25 yr) were selected from qualified subjects and randomly assigned to four groups: placebo (n=10), supplement (n=10), training (n=10) and training+supplement (n=10). The training program included 8 weeks of rope training (four sessions per week and 45 minutes each session), and daily supplementation of 1200 mg portulaca for 8 weeks. The variables were measured by ELISA method. The data were analyzed by ANOVA test at a significance level (P<0. 05). The results showed that the amounts of MAD in training+supplement and training groups decreased significantly (P=0. 01, P=0. 001) and SOD, GPX and TAC increased significantly in training, supplement and training+supplement groups [SOD (P=0. 001, P=0. 03, P=0. 001), GPX (P=0. 02, P=0. 007, P=0. 004), TAC (P=0. 02, P=0. 01, P=0. 001) respectively]. It seems that incremental interval endurance training as rope training along with portulaca supplementation can improve the status of antioxidant system and reduce oxidative damage and may influence preventing cardiovascular diseases.