In order to investigate the effect of phosphorous and potassium bio mass fertilizers on some important morphological traits, agronomic characteristics and yield of red bean, the present experiment was conducted at Agricultural Research Farm of Islamic Azad University, Arak, in the summer of 2013-2014in two years. The research treatments were conducted as split plots based on randomized complete blocks design with three replications. Experimental treatments included the use of bio fertilizers at four levels of non-use, application of phosphorus bio fertilizer, potassium bio fertilizer and phosphorus bio fertilizer + potassium bio fertilizer and four red bean genotypes, by the names of Goli, D81083, Derakhshan, and KS31169/3. The application of bio fertilizers was carried out early in the vegetative period and also in the pre-flowering stage. The results showed that application of biological fertilizers could significantly affect the yield of single plant, number of seeds per plant, number of pods per meter square, number of sub branches, chlorophyll a, Chlorophyll b, chlorophyll a to chlorophyll b ratio, the shell weight to the weight of the entire pod ratio and potassium to seed phosphorus ratio. In this investigation, Goli cultivar produced the highest seed yield of 71/8 gram per plant Also, on average the grain yield of beans increased by 25. 5%. from 56. 6 gram per plant in the absence of bio fertilizers to 80. 8 gram per plant in combined fertilizer application. The investigated genotypes showed significant difference in all of the studied traits, but the interaction between application of fertilizers and genotype was significant only in number of seeds per plant, number of pods per square meter. Thus, using bio fertilizers, in addition to quantitative increase and absorption of essential elements by stimulating growth, can improve quality characteristics of red beans.