Drought stress is the most prevalent abiotic stress in Iran and has highest negative effect on plant growth and productivity. Polyamines are plant growth regulators that enhance resistance of plants to environmental stresses such as water deficit. In this research, the effect of different putrescine+spermine concentrations (0+0, 40+40, 40+60, and 60+40 μ M) on some of the physiological characters of spring safflower under well-watering (100% FC) and limited-watering (40% FC) were studied. The experiment was arranged as factorial based on randomized complete block design with three replications in a greenhouse at the University of Tabriz. Without polyamines application, water deficit was decreased growth parameters, leaf relative water content, photosynthetic pigments, and soluble protein in the leaves. Proline and soluble sugar contents of leaf were increased under water deficit. Foliar application of 60+40 μ M putrescine+spermine improved most of these traits in the leaves, particularly under water stress conditions. However, exogenous polyamines reduced leaf proline content probably due to increase in chlorophyll content. Reduction in compatible osmolytes such as free proline in root and soluble sugars in leaf with application of 40+40 and 40+60 μ M putrescine+spermine could be due to polymines role as osmolyte as well as ROS scavenger. These results suggest that foliar spray of 60+40 μ M putrescine+spermine can mitigate some of the harmful effects of drought stress on physiological parameters of safflower.