Effect of explant types and different levels of plant growth regulators on callogenesis and regeneration in medicinal plant, Matricaria aurea

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Vatandoost SH.; ZEBARJADI A.R.

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Journal: Journal of Plant Research Year:2020 | Volume:33 | Issue:1 Page(s): 00-00

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Title

Effect of explant types and different levels of plant growth regulators on callogenesis and regeneration in medicinal plant, Matricaria aurea

Author(s)

Vatandoost SH. | ZEBARJADI A.R. | Issue Writer Certificate

Keywords

Matricaria aureaQ2

Indirect regenerationQ1

CallusQ2

NAAQ1

BAPQ1

Abstract

Matricaria aurea is an important medicinal plant belongs to Asteraceae family, which is used widely in pharmacy, food industry, and cosmetics purposes. Regarding importance of this medicinal plant, optimization of its tissue culture could be predisposing factor of many types of research about the usage of this plant. Callus induction and Indirect regeneration were applied for optimization of M. aurea tissue culture. Both experiments were conducted in a randomized complete design that was arranged as 2×4×4 factorial with 3 replications on MS medium. For both experiments, various levels of NAA and BAP were compared along with two explants (stems and leaves). The result showed that there was no Callus induction observed in growth hormone-free medium. The result showed that Callus induction for both explants was faster in 0. 5 mg/l NAA+1 mg/l BAP. Two hormone compositions: 0. 5 mg/l NAA+1 mg/l BAP and 1 mg/l NAA+1 mg/l BAP were the best composition for Callus induction of both stems and leaves. The highest Callus diameter (15. 31 mm) obtained with 2 mg/l NAA + 1. 5 mg/l BAP on leaves. Also, highest Indirect regeneration (85. 59%) was observed in 0. 5 mg/l NAA + 1 mg/l BAP on calli that were obtained from leaves.

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APA: Copy

Vatandoost, SH., & ZEBARJADI, A.R.. (2020). Effect of explant types and different levels of plant growth regulators on callogenesis and regeneration in medicinal plant, Matricaria aurea. JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 33(1 ), 00-00. SID. https://sid.ir/paper/360485/en

Vancouver: Copy

Vatandoost SH., ZEBARJADI A.R.. Effect of explant types and different levels of plant growth regulators on callogenesis and regeneration in medicinal plant, Matricaria aurea. JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2020;33(1 ):00-00. Available from: https://sid.ir/paper/360485/en

IEEE: Copy

SH. Vatandoost, and A.R. ZEBARJADI, “Effect of explant types and different levels of plant growth regulators on callogenesis and regeneration in medicinal plant, Matricaria aurea,” JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 33, no. 1 , pp. 00–00, 2020, [Online]. Available: https://sid.ir/paper/360485/en

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