Identification of a protein that inhibit amylase activity in seed of wheat

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HeidariZadeh M.; HASANVAND F.

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Journal: Journal of Plant Research Year:2020 | Volume:33 | Issue:1 Page(s): 00-00

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Title

Identification of a protein that inhibit amylase activity in seed of wheat

Author(s)

HeidariZadeh M. | HASANVAND F. | Issue Writer Certificate

Keywords

wheatQ2

enzyme inhibitorsQ1

alpha-amylaseQ1

Abstract

Storage proteins which found in seeds of many plants are studied in various fields such as human and animal nutrition, chemistry of protein, pharmacology, plant biochemistry and medicinal plants. The ability of these compounds to cause nutritional problems and toxic effects when used as foods has led to several studies on their dispersion in plants. In this research, seed proteins of wheat (Zarrin variety) have been extracted. The protein of interest was purified by ammonium sulfate precipitation method; dialysis and ion exchange chromatography. After purification by Fast protein liquid chromatography (FPLC), electrophoretic properties of this protein were investigated by Sodium dodecylpolyacrylamide gel electrophoresis (SDS-PAGE) method. Inhibitory activity of this Protein against Bacterial alpha-amylase and human saliva were measured using the Bernfeld method. FPLC diagram illustrates the purification and collection of the desired protein. The electrophoretic pattern of this protein with relative mobility of 0. 60 and 0. 59 confirmed the purification process. Hydrolytic activity of bacterial and human saliva alpha-amylase decreased by this protein 89. 97% and 97. 07% respectively. In general the isolation, purification and alpha-amylase inhibition property of this protein which extracted from wheat seed were confirmed in this study. Inhibition of bacterial alpha-amylase by this protein can be considered by agricultural researchers in biological control of parasites and pests. This alpha-amylase inhibitor can also be used to treat diabetes, digestive deficiencies and modify dietary regimens to reduce weight.

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APA: Copy

HeidariZadeh, M., & HASANVAND, F.. (2020). Identification of a protein that inhibit amylase activity in seed of wheat. JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 33(1 ), 00-00. SID. https://sid.ir/paper/363112/en

Vancouver: Copy

HeidariZadeh M., HASANVAND F.. Identification of a protein that inhibit amylase activity in seed of wheat. JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2020;33(1 ):00-00. Available from: https://sid.ir/paper/363112/en

IEEE: Copy

M. HeidariZadeh, and F. HASANVAND, “Identification of a protein that inhibit amylase activity in seed of wheat,” JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 33, no. 1 , pp. 00–00, 2020, [Online]. Available: https://sid.ir/paper/363112/en

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