Purpose: The use of nutritional interventions such as dietary supplements is recommended to prevent early onset of fatigue, physical activity stresses, and improved performance during the race. The aim of this study was to examine The effect of four weeks HIIT training with ginseng supplementation on aerobic, anaerobic powers and body composition of Overweight and obese females. Methods: In this semi-experimental study, 40 overweight-obese women with mean and standard deviation were weight 77. 30 ± 9. 97 kg, age 33. 01 ± 3. 5 years old, and Body fat percent %36. 39± 3. 7, which is purposefully, grouped in four supplements, supplement-training, training and control groups. The HIIT training protocol lasted for four weeks and three sessions a week, and each session was 4-6 sets and each set was 30 seconds, fast paced with 80% MHR and 270 seconds fast walking for the training groups. Each participant in supplement and supplement-training groups has taken one 500 mg ginseng supplement capsule for four weeks each day. The fat percentage was measured by the Jackson-Pollack three-point method and peak power output (PPO), maximum power point (MPP), and fatigue index (FI) measured by the 30-Seconds Wingate test and Vo2max by Queen’ s test method. The data were analyzed with covariance analysis with using SPSS22 software at a significance level of 0. 05. Results: As the results of covariance analysis showed that Ginseng supplementation with HIIT training on maximum aerobic power, Peak anaerobic power, average anaerobic power, minimum anaerobic power, weight, fat percentage, WHR, there is significant difference between four groups (p<0. 05); but there is no significant difference between four groups in LBM (p>0. 05). Conclusion: Intense interval training with ginseng supplementation improves aerobic and anaerobic capacity and body composition (body fat percentage, weight, WHR and BMI) of women with overweight and obesity.