JOURNAL OF CELL & TISSUE
Year:2019 | Volume:10 | Issue:2|Papers Count:6

Aim: In this study the protective effects of quercetin were investigated on myocardial tissue damage in male Wistar rats during malathion poisoning. Material and Methods: The study was performed on seven groups; each group contained six male rats. After 24 h of intraperitoneal injection of quercetin, malathion or a combination of them, the heart of the animals was separated, and its tissue slice was prepared. After tissue homogenization, the parameters of oxidative stress were measured. Results: Intraperitoneal injection of 200 mg/kg of malathion significantly induced the lipid peroxidation (p<0. 01) and oxidative stress (p<0. 001), necrosis and inflammation in myocardial tissue. However, intraperitoneal injection of 50mg/kg quercetin reduced malathion-induced toxicity on lipid peroxidation (p<0. 001), oxidative stress (p<0. 05), inflammation and myocardial tissue damage. Conclusion: The results suggested that quercetin is able to improve myocardial tissue damage and restore the rate of oxidative stress in malathion-treated groups in the normal level.

Aim: The aim of this study was the effect of zolpidem on oogenesis and FSH, LH hormones of female adult NMRI mouse. Material and Methods: In this experimental study thirty adult female mice NMRI strain at a mean weight of 30± 26 grams were divided into five groups Zolpidem solution was prepared in distilled water at 5, 10, and 20 (mg/kg of body weight) doses, and 0. 5 cc injections were doneintraperitoneally every day for 14 days The control group received no injection. The sham group received distilled water and treatment groups of 1, 2, and 3 received doses of 5, 10, and 20 mg/kg The treatment groups were sacrificed one day after the last injection The concentrations of the hormones were measured by the ELISA test The ovary tissue was separated and examined as well as Hematoxylin and eosin painting and the results were evaluated via the tukey-test, (One-way ANOVA) by SPSS program. Results: The results showed a significant decrease in the mean serum FSH, LH levels in the experimental groups compared to the sham and control (P<0. 05) Also histological studies of sections showed the mean number of graffian, secondary and corpus luteum reduced and the mean number of primordial, growing, atretic and abnormal follicles increased. In addition, a significant reduction in the diameter of secondary, graffian follicles and corpus luteum and a significant increased in the diameter of primary, atretic follicles were observed in the experimental groups, compared to the sham and control groups. Conclusion: Injection of zolpidem significantly is effective on, oogenesis and concentration of LH, FSH hormones.

Aim: The aim of this study is determining the sensitivity and specificity of simultaneous use of both DNA methylation of RASSF1A gene and expression of FHL1 gene in the differential diagnosis of benign tumors from malign papillary carcinoma tumor in the thyroid gland. Material and Methods: 160 samples of patients with malign thyroid tumors (80 samples) and benign thyroid (80 samples) were used in this study. The RNA was extracted from paraffinic samples, and then cDNA was made from it. Gene expression was investigated by Real Time PCR mixture containing the Cyber Green fluorescence material. After DNA extraction, the hypermethylation of the gene was performed using COBRA method. Finally, an epidemiological calculation was used for calculating the effects of sensitivity and specificity of two simultaneous tests. Results: There was a negative significant correlation (P ≤ 0. 05) between promoter hypermethylation of the RASSF1A gene and the FHL1 gene expression. Net sensitivity of simultaneous use of the promoter methylation of the RASSF1A gene (quantitative and quantitative) and expression of the FHL1 gene were 98. 2% and 79. 32%, respectively. The Net specificity of simultaneous use of the promoter methylation of the RASSF1A gene (quantitative and quantitative) and the FHL1 gene expression test were 39. 47 and 75. 29%, respectively. Conclusion: simultaneous use of the hypermethylation of the RASSF1A gene promoter as a qualitative and expression test for FHL1 gene is more sensitive and more reliable in differential diagnosis of benign tumors from malign papillary carcinoma tumors.

Aim: Wallerian degeneration is an innate immune response which occurs after nerve injury. In this study, the effects of dexamethasone (Dex) and thiamine (Thi), as anti-inflammatory drugs, were compared on the Wallerian degeneration. Material and Methods: The right sciatic nerve of 45 male rats was transected and the rats were divided into five groups (n=9). Animals in groups 2 to 5 daily received (i. p) low and high doses of Dex and Thi up until sampling days, respectively. Group 1 was considered as control. At 5th, 7th and 10 th days after axotomy, 3 rats from each group were killed and the distal stump of sciatic nerve was served for histological assessment. Results: In the histological examinations of all groups, edema, degraded myelin sheets and some infiltrated mononuclear cells were observed. But administration of high doses of Dex and Thi (groups 4 and 5) alleviated all the above mentioned histological changes. In comparison with control group, the groups received high doses of Dex and Thi showed significant increase in the number of myelinated nerve fibers (p<0. 05). Measuring of the cross surface area of the sciatic nerve showed no significant differences. Finally, administration of high dose of Dex was associated with several side effects. Conclusion: Although these findings indicated the beneficial effects of both Dex and Thi but due to the severe adverse effects of Dex, it is recommended to prescribe Dex for acute and short term cases of nerve injuries, whereas in the case of chronic and long term inflammatory conditions Thi administration may be preferred.

Aim: The aim of this study was purification of magnetosomes of Magnetospirillum gryphiswaldense and investigation cytotoxicity effect of the purified particles of this bacterium on the breast cancer cell lines. Material and Methods: In this study, the standard strain of bacteria was cultured on DSMZ broth at temperature of 28° C for seven to ten days and incubated under the microaeraphilic conditions. To ensure phenotype tests, the PCR reactions were performed using specific primers (MT1166), associated with scanning electron microscope imaging. The influence of purified magnetosome from Magnetospirillum gryphiswaldense in various concentrations was investigated on the breast cancer cell lines. Kruskalwalis test was performed for statistical analysis. Results: Gram staining results showed that the bacterium was gram negative spirillum. Electronic micrographs indicated that the magnetosome of this bacterium was 60 nanometer in size. Purified magnetosome from Magnetospirillum gryphiswaldense did not show considerable cytotoxic properties on the breast cancer cell lines. The percentage of living cells against magnetosomes purified from the bacterium Magnetospirillum gryphiswaldense was 85% (p=0. 04). Conclusion: Magnetospirillum gryphiswaldense magnetosomes killed 15% of the cancer cells. The size and structure of magnetosomes of Magnetotactic bacteria are great importance in the elimination of the breast cancer cells.

Aim: Evaluation effects of ZnO nanoparticles on peroxidase activity, gene expression of peroxidase enzyme, proline content and expression of proline-5-carboxylate synthetase (P5CS) gene in L. draba seedlings in compared to the effects of its balk form. Material and Methods: L. draba seedlings were grown in the presence of different concentrations of these particles for 7 days in fully-random designs with three repetitions; thereafter the mentioned parameters were measured. Results: While the peroxidase activity was significantly evaluated in the presence of nano and balk particle in the concentration more than 100 and 50 mg/L respectively, in them, the gene expression level of peroxidase was the same as the control. On the other hand, in the treated seedlings, proline content was significantly increased in accordance to the rising nanoparticle doses in media, while, no significant difference was seen in the P5CS gene expression in different treatments rather than control sample. In the balk treated seedlings, content of this Abstract amino acid were significantly increased up to 100 mg/L concentrations and dramatically decreased in the presence of doses more than 250 mg/L in compared to the control sample. Gene expression level of P5CS was the same as the control in the presence of the balk form up to 500 mg/L and significantly decreased at the highest concentration. Conclusion: Based on the results, it seems that nanoparticle has fewer effects rather than the balk form on the mentioned parameters, which may be due to release less Zn ion in this condition.