Transformation requires innovative tools to monitor and manage organizational traumas and diseases. This research has been done with an innovative pathological approach and with the aim of recognizing and managing one of the organizational diseases, called "organizational stroke", by constructing and validating its measurement tools. To conceptualize organizational stroke, the qualitative methodology of content analysis methodology, Attride-Stirling Approach, has been used and to validate the organizational stroke measurement instrument, the quantitative correlation methodology has been employed. To ensure the validity of the constructed instrument, 13 public organizations of Hamadan province have been considered as the statistical population of the research. A total of 583 employees were selected as the sample by cluster sampling method. For data analysis, the partial least squares method and Smart PLS2 software were used. The validity of the questionnaire was calculated based on face validity, content validity, and structure validity, and its reliability was calculated based on Cronbach's alpha method and combined reliability. The final designed instrument includes 92 items in three dimensions of antecedents (lack of openness, disagreement, lack of support, lack of individual homogeneity, negativity, lack of education, lack of skills); examples (structural, moral, behavioral, strategic, cognitive, belief, information, knowledge, environmental strokes), and consequences (structural, behavioral, social, psychological traumas). It is recommended to use this measurement instrument to identify the extent to which the organizations are suffering from organizational stroke and the endeavor to control it.