Hormozgan Medical Journal
Year:2013 | Volume:16 | Issue:6|Papers Count:11

Introduction: Mammalian cells culture is an important method for producing recombinant proteins, monoclonal antibody, and hormones application. One of the common methods in the monocyte isolation is using flask adherent cell culture in RPMI-1640 medium. We aimed, in this study, cell culture medium using dextrose 5% with autologous plasma to produce inexpensive medium for isolation of monocytes.Methods: In this experimental study, we isolated peripheral blood mononuclear cells from 40 healthy volunteers, the isolated cells were cultured in RPMI 1640 medium containing 10% fetal calf serum and in the media supplemented serum dexterous 5%containing autologous plasma under 37oC and 5% CO2 in a cell incubator. Then, count, viability and morphological cells were surveyed by trypan blue and invert microscope.Results: We isolated 1.8-2´106 cells /ml of RPMI culture media and 2-2.2´106 /ml macrophage of serum dexterous 5% containing autologous plasma. There was not a significant difference between two methods (P>0.05). Also, we did not see any morphological variation in two culture media.Conclusion: Our results showed that dextrose 5% can be used in monocyte isolation from peripheral blood mononuclear cells.

Introduction: Tubal Sterilization (TS) is one of the most common contraceptive methods. Some researchers believe that TL has complications, whereas there are contradictory reports about TS effects on ovarian function. The aim of this study was to evaluate the indices of oxidative stress and survey ovarian function after TS.Methods: The study groups consisted of 63 Sprague Dawley female rats. The study comprised of 2 protocols A and B. A Protocol: survey oxidative stress and ovarian hormones and B protocol: study of estrous cycle. Each of the protocols included control, sham and Tubal Sterilization (Pomeroy) groups. In protocol A, rats of PAB (Prooxidant- Antioxidant Balance), MDA (Malondialdhyde) 17 beta estradiol and progestrone were measured 15 and 45 days after intervention. In protocol B, 15 and 45 days after intervention, animal’s estrous cycle was followed for 15 days. Data were analyzed by means of SPSS. Software, using ANOVA and P<0.05 was considered statistically significant.Results: Our results showed that PAB and MDA significantly increased and estradiol and progesterone significantly decreased in rats undergone TS (P<0.05). Also TS caused irregularity in estrous cycle after 15 and 45 days.Conclusion: TS Increased oxidative stress level and decreased estradiol and progesterone of serum. Complications of TS may be due to increased oxidants and decreased estrogen and progesterone. By prescribing antioxidant, effects of TS can be reduced.

Introduction: Heavy metals as environment pollutant are potentially dangerous for living organisms. These metals cause interruption of enzyme activity and impaired synthesis of compounds that are essential for body, and can accumulate in aquatic bodies. The aim of this study was to determine lead, cadmium, nickel and zinc in canned fish in south of Iran.Methods: In this descriptive and analytical study, sixteen different brands of canned tuna were collected during a two months period from April to May 2010 in south of Iran. Three cans from each brand were purchased and one sample was taken from each brand. Totally, 192 experiments were performs on samples. Sample preparation and the digestion rate of heavy metals including lead, cadmium, nickel and zinc was determined by flame atomic absorption value. Results were analyzed using descriptive statistics by SPSS Software.Results: Concentrations of lead, cadmium, nickel and zinc in collected canned tuna, were 0.2115±0.11, 0.3514±0.014, 0.4098±0.24 and 0.77±00.36 mg/g, respectively.Conclusion: Our results showed, that the average concentration of heavy metals was lower than those declared by FAO and WHO. Given the importance of heavy metals on human health, periodic monitoring of these metals is necessary.

Introduction: The silent brain lesions detected by MRI were fairly common not only in first-ever stroke but also in normal elderly subjects. Some recent studies show the possible role of silent sub-cortical brain infarction in ischemic stroke. The aim of this study was to evaluate the frequency of silent sub-cortical brain infarction in acute first-ever ischemic stroke.Methods: In this descriptive study, frequency of silent sub-cortical brain infarction was determined with brain MRI in 200 patients with acute first-ever ischemic stroke. Furthermore, demographic features and frequency of risk factors of ischemic stroke were evaluated. The data were analyzed by SPSS software version 16, using of t-test and Fisher Exact test.Results: In this study, 43% of our participants were men and the rest were women. The mean age of men was 68.75±8.53 and the mean age of women was 66.15±12.45.38.5% of patients with acute ischemic stroke had silent sub-cortical brain infarction. There was no significant relationship between silent sub-cortical brain infarction and risk factors of ischemic stroke.Conclusion: The frequency of silent sub-cortical ischemic brain lesions was high in ischemic stroke. Silent sub-cortical ischemic brain lesion may be a risk factor for ischemic stroke.

Introduction: Ovarian cancer is the 6th most common cancer and the 7th cause of death from cancer in women all around the world. The aim of this study was to determine the related factors with survival of ovarian cancer among women in Fars Province, southern Iran.Methods: In a survival analysis study, the recorded data at cancer registry center of Shiraz university of medical sciences related to women with ovarian cancer between years 2002- 2009 were studied. Variables such as residency, marriage status, occupation, religion, age, metastasis, abortion history, stillbirth history, menarche age, menopause age, prime gravid age, literacy, smoking, BMI and breast feeding history were collected from the cases.Results: Out of 201 women with ovarian cancer 22 cases died during the study with average survival rate of 86.14 months.101 cases were residing in Shiraz city and 100 cases in other towns and villages. Marriage status, menarche age, abortion history, breast feeding history, metastasis and pregnancy history indicated relationship with death odds, whereas, in multiple analyses, only three of them (menarche age, metastasis and abortion history) indicated relationship with death odds. The results also showed a slight increase in both adjusted and unadjusted cumulative survival probability of 1, 2 and 3 years.Conclusion: In general, it may be concluded that, cumulative survival probability among patients of Fars province is comparatively higher than other areas.

Introduction: Cutaneous Leishmaniasis is transmitted to humans mainly by Phlebotomus papatasi and P. sergenti sand flies in Iran. This survey was conducted in order to determine species composition and abundance of sand fly species in Saravan County Iran.Methods: This field study was conducted in 1997-1998. Sand flies were collected from outdoors by sticky paper traps. Identifications were based on the morphology of the male genitalia and female spermatechae and pharynges using standard keys.Results: Based on the results, S. tiberiadis (18%), P. alexandri (16.5%) and P. kazeruni (14.3%) were found to be the most abundant species. Other species were P. sergenti, S. clydei, S. hodgsoni, P. papatasi, S. sintoni, S. christophersi, S. baghdadis, S. iranica, S. mervynae, P. eleanorae, P. bergeroti, S. dreyfussi, S. dentata, P. mesghali, S. grekovi, S. theodori and S. africana, respectively. During the study period, the species of P. eleanorae, P. bergeroti, P. mesghali, S. hodgsoni, S. christophersi, S. mervynae, S. dreyfussi, S. dentata, S. grekovi, S. africana and S. theodori were captured for the first time from this county.Conclusion: In this study, P. papatasi, P. sergenti and P. alexandri comprised 69.6% of catches during the study period. These species are the proven vectors of Leishmania major, L. tropica and L. infantum in Iran, respectively.

Introduction: The occurrence of Low Back Pain (LBP) among children has almost reached the level in adult, and it is becoming a serious health concern in school-age children. Few studies documented prevalence of LBP in children in Iran. The purpose of this study was to determine the prevalence of LBP and associated risk factors in school-age children.Methods: After ethical approval, a cross-sectional study was conducted on 1648 randomly recruited schoolchildren aged 7-15 years in the south of Iran. A structured questionnaire was used to collect the data. The data were analyzed using logistic regression by SPSS software.Results: Based on the results, 1243 children reported LBP point and last month prevalence of LBP in this study was 50% and 48%, respectively. Logistic regression model showed that age, time of watching television, type of bag and position of homework are significant risk factor for LBP in children.Conclusion: The results indicated that the prevalence of LBP in schoolchildren is higher than the normal rate. Some of risk factor significantly influenced LBP in children.

Introduction: The aim of this study was to evaluate the need for revision of the undergraduate pediatric dentistry curriculum of Shiraz dental school according to the opinions of the professor and students.Methods: This cross-sectional study was performed in academic year of 2008-2009.77 questionnaires distributed between 8 pediatric professors and 69 students. Questionnaire in theoretical pediatric and practical pediatric dentistry was designed according to the likert criteria. The data was analyzed by SPSS-14 and descriptive statistics and Mann Whitney test was used for statistical analysis P<0.05 was considered statistically significant.Results: In comparison with the three theoretical courses, course 2 was the most important and course 3 had the least importance. Only in course 2, the statistical difference was significant (P=0.017). It was shown that, some topics were common with course topics in other departments. Educational titles for courses 1 and 2 overlapped with the operative dentistry and orthodontics and course 3 is mostly overlapped with oral medicine subjects. Pulp and fissure sealant therapy were the most important topics in practical pedantic dentistry.Conclusion: There is a need to revise and modify the theoretical and practical pediatric dentistry educational program.

Introduction: Family violence is an aggressive behavior happening among some couples. According to WHO reports, familial violence against women is a health priority and has many complications. This study investigated the physical, mental and sexual violence exposure among the married women in reproductive age in Zahedan, Iran.Methods: This cross sectional study carried out on 354 married women living in Zahedan city in 2009. The data was collected through interview and by using a valid and reliable questionnaire. At first, the subjects were categorized based on violence exposure level, and then the data were analyzed using descriptive statistic and chi square test. P-value<0.05 was considered statistically significant.Results: The results showed that the exposure prevalence with physical, mental and sexual violence was 5.4%, 20.9% and 9.7%, respectively. The educational level of husband, number of children and age of marriage were related to all three types of violence (P<0.04) and ethnicity, educational level and husband’s job were related to physical and mental violence (P<0.05). Additionally, mental and sexual violence were associate with monthly income of family (P<0.05).Conclusion: It seems that violence exposure among Zahedanian women is more than expected. That is probably due to low Socio-economic statues and illiteracy.

Introduction: Chlorination has been used as a major disinfectant process for public drinking and swimming pool water in many countries. However, it was concern over the disinfection byproducts (DBPs) by the reaction of chlorine with organic material in water and the effect on human health. The purpose of this study was to determination trihalomethane concentrations in indoor swimming pools water of Bandar Abbas.Methods: Water samples were collected from five different indoor swimming pools in Bandar Abbas. A total of 15 pool samples were collected and analyzed for determination of chloroform, TOC, EC, Turbidity. Trihalomethane (chloroform) analysis by the GC system was performed with ECD detector. Water and air temperature and the amount of residual chlorine and PH levels were measured at the site.Results: In this study, mean concentration of chloroform in swimming pool water was 93.48 micrograms per liter and the mean concentration of total organic carbon (TOC) in swimming pool was 9.58 milligrams per liter. There was a correlation between chloroform and TOC (r=0.49, P<0.05), between number of swimmer and chloroform (r=0.636, P<0.01) and between residual chlorine and chloroform (r=0.639, P<0.01).Conclusion: High concentration of trihalomethane was found in Bandar Abbas swimming pools. The difference between the number of swimmers, water temperature, residual chlorine, and TOC influence on Trihalomethane Concentrations between pools is more than within pools.