The present research aim to evaluate psychometric properties, validity and reliability of Minnesota eating behavior survey (MEBS, von Ranson, Klump, Iacono, Mc Gue, 2005) in female high school students in Isfahan .This scale is one of the self-report tools for evaluation of anorexia nervosa symptoms. After translation the English version to Persian and back translation of MEBS, the existed problems in accordance of two translation were considered and corrected, so it was performed in a small sample for identification of probable problems and after that, the essential corrections were done and the final inventory was administered. Participates of the study were 300 female high school students from the five regions of Esfahan the samples were selected through multi-stage cluster random sampling. They fill MEBS and the data were analyzed with SPSS and LISREL. Factor analysis of the scale via the varimax rotation showed 3 factors, that covered 56.83 percent of the total variance. Construct validity also accepted through the 3 factors. Also data analysis reported a relatively high internal consistency (cronbach alpha was 0.70).The correlation coefficient between test and retest were 0.93 that indicated a high test-retest reliability. To consider range of point, T scores was used and score 19 was indicated as the border of normal from abnormal diagnosis. The results show that MEBS is a valid and reliable scale that can be apply in research and clinical situations to diagnose the symptoms of anorexia nervosa in female adolescents of Esfahan.