Heavy mortalities occur during the critical period of early stages of the Persian sturgeon, A. persicus, development. These mortalities may be caused by yolk sac absorption, endogenous feeding change to exogenous, incomplete development of immune system, etc. This study was performed to determine the effects of levamisole as an immunostimulator agent on mortality reduction in the Persian sturgeon fry. The experiment included one control and two treated groups in which 5 and 10 mg/l levamisole were applied on daily bath for 20 min. In order to determine the days of critical period during the early stages of development and to study the levamisole effect on mortality reduction, daily mortality percentage was calculated in treatment and control groups. The results showed that critical period in the Persian sturgeon was on days 11 to 13 after hatching. In this investigation, early mortality rates were 6%, 4% and 3% in the control, 5 and 10 mg/lit levamisole treated groups, respectively. The survival rate was 71.05%, 76% and 87.05% in the control, 5 and 10 mg/lit levamisole treated groups, respectively. The result showed that levamisole was capable to decrease mortality rate and increase survival in fry of sturgeon significantly (p<0.05).