Introduction & Objective: More than half of made sexual problems that because of insufficient knowledge lead to unsafe marriage relationship. By considering the sexual and reproductive health education role in preventing sexual problems and health promotion, the aim of this paper was studying the effect of the education based on James Brown pattern on knowledge of sexual and reproductive health in women participant marriage educational classes.Materials & Methods: The Quasi-Experimental study conducted on 330 Hamedanian women in case and witness groups (randomly selected) in 1386. In case group, education was done based on James Brown education pattern and the witness group took part in education class in clinic. Data analysis was done by SPSS11 software, Chi-square and T. test formulas. The amount of meaningful statistical was P<0.05.Results: Knowledge average scales in case group, including sexual health, anatomy and physiology, diverse aspects of sexual health and awareness of AIDS, STDs and family planning, after education with James Brown pattern increase (81.7, 78.6, 84.0, 85.9) rather than pre-education stage (44, 45.8, 49.3, 43) (P=0.00). While knowledge average scale in witness group before and after education has no meaningful statistical difference (P=0.62).Conclusion: According results Hamedanian women knowledge was weak. James Brown pattern makes increasing of their awareness about sexual health. So it's suggested to generalize the reproductive and sexual health education by James Brown education pattern to Hamedanian women and other Iranian women.