Aspects of the biological parameters of local populations of the sweet potato whitefly, B. tabaci (Genn.) (Hom: Aleyrodidae) as an important pest of cotton were compared during 2001. The infested cotton leaves containing nymphs and pupae were collected from Darab, Qom, Saveh, Gonbad, Gorgan, College of Agriculture campus, Varamin, Garmsar, Orsoiieh (Kerman), Rafsanjan and Shooshtar fields. Experiments were conducted in a growth chamber under 24±20C, 55±3% RH and 16:8 (L:D) photoperiod on cotton, Gossypium hirsutum L. (Varamin 76 variety). The newly emerged populations of each locality were released into a large cage (40x50x70 centimeter) set on cotton plants separately. Emergence of adults, and crawlers and percentages of females emerged from 100-200 eggs at four replications were also calculated. Total fecundity, oviposition period, adult's longevity, sex ratios and daily fertility rates of 50 mated females were calculated for each local population. The grouping results indicated that total eggs/female for B. tabaci populations were ranked highest in Saveh, Collage of Agriculture and Qom (113.25, 102.63 and 92.88) respectively, medium in Garmsar, Shooshtar, Orsoiieh, Gorgan, Rafsanjan and Gonbad (89.75, 82.63, 73.63, 63.88, 63.5 and 61.38) respectively and lowest at Darab (57.88). There were significant differences between parameters of total fecundity and oviposition period at 5% level and between sex ratios and developmental time at 1%. Level. However, no significant difference was found between maximum adults longevity at 5% level.