Objectives: This study investigated the effect of acute resistance exercise on brain-derived neurotrophic factor (BDNF), Insulin-like growth factor (IGF-1) and its receptor (IGFBP-3) in the elderly.Methods & Materials: 22 healthy older men participated in this study (age range: 60-75 years). 72 hours after determining muscular maximal strength (by 1-RM test), the elderly participated in acute resistance exercises via 75% 1-RM. Three blood samples were collected at baseline, immediately and 4 hours after the exercise from the Antecubital vein. Serum BDNF, IGF-1 and IGFBP-3 were measured by ELISA methods. Also, for statistical analyses Pearson correlation test and Repeated Measures (1×3) were used. The significance level was set at P≤0.05.Results: The results showed significant increase in serum BDNF levels, immediately after a session of resistance exercise. But 30 minutes after completion of the protocol, there were no significant changes compared with baseline values (P≤0.05). There was also a significant increase in the amount of IGF-1, after a session of resistance exercise. But 30 minutes after completion of the protocol, there was no significant change compared with baseline (P≤0.05). In the end, there was no significant change in the levels of IGFBP-3 in immediately and 30 minutes after resistance exercise protocol, compared with baseline. Conclusion: In response to resistance exercise, the amount of BDNF and IGF-1 significantly increased, immediately after the exercise, but there was no significant change in the levels of IGFBP-3. It seems that resistance exercise causes positive changes in the amount of neurotrophic factors involved in learning and memory. Subsequently, it may causes reduction in the prevalence of neurological disorders associated with learning and memory impairments such as Alzheimer’s, depression, and dementia in the elderly.