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Information Journal Paper

Title

Rapid Homemade Reverse Transcriptase PCR Detection and Phylogenetic Analysis for SARS-CoV-2 Based on E and M genes

Pages

  187-193

Abstract

 Background & Objective: Coronavirus disease 2019 spreads worldwide and needs Detection systems capable of rapid diagnostic of this virus (SARS-CoV-2). The aim of this study is to design the homemade RT-PCR method for the Detection and Phylogenetic Analysis of this virus. Material & Methods: The genes selected for diagnosis were E and M genes for this virus. PCR product was cloned in pTZ57R/T plasmid for preparation of positive control. In order to determine the sensitivity of this molecular method, the genes mentioned in the clone pTZ57R/T vector and the Limit of Detection (LOD) the genes were determined and Phylogenetic Analysis was performed using partial E and M gene sequences. Results: PCR product was observed for E and M genes 156 and 547 bp on the Agarose gel. The LOD of the E and M gene was 60 and 82 copies. There was also a positive response to the samples of patients who were positive by other methods. Conclusions: Since this virus is considered to be the cause of a pandemic in different countries all over the world, the present study is very important as a method of rapid and low-cost molecular diagnosis for monitoring this virus. Phylogenetic Analysis is necessary for epidemiological studies for the control and prevention of the disease.

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    Cite

    APA: Copy

    MOROVVATI, ABBAS, JAVADI, ALI, Dehghani Sanij, Somayeh, ZAREI, MOHAMMAD REZA, Badakhsh, Mina, Zargar, Mohsen, & Molazadeh, Shima. (2022). Rapid Homemade Reverse Transcriptase PCR Detection and Phylogenetic Analysis for SARS-CoV-2 Based on E and M genes. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, 12(2), 187-193. SID. https://sid.ir/paper/1000405/en

    Vancouver: Copy

    MOROVVATI ABBAS, JAVADI ALI, Dehghani Sanij Somayeh, ZAREI MOHAMMAD REZA, Badakhsh Mina, Zargar Mohsen, Molazadeh Shima. Rapid Homemade Reverse Transcriptase PCR Detection and Phylogenetic Analysis for SARS-CoV-2 Based on E and M genes. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES[Internet]. 2022;12(2):187-193. Available from: https://sid.ir/paper/1000405/en

    IEEE: Copy

    ABBAS MOROVVATI, ALI JAVADI, Somayeh Dehghani Sanij, MOHAMMAD REZA ZAREI, Mina Badakhsh, Mohsen Zargar, and Shima Molazadeh, “Rapid Homemade Reverse Transcriptase PCR Detection and Phylogenetic Analysis for SARS-CoV-2 Based on E and M genes,” JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, vol. 12, no. 2, pp. 187–193, 2022, [Online]. Available: https://sid.ir/paper/1000405/en

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