Analysis of the Expression of Dopamine Receptors, DRD1 and DRD5, in Cancerous Tissue of Patients with Breast Cancer and the Impact of Quercetin on the Expression of These Receptors in Hs578t and HDF Cell Lines

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Kiani Rad Kowsar; RIGI GARSHASB; REIISI SOMAYEH

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Advanced Biomedical Sciences Year:2022 | Volume:12 | Issue:2 Page(s): 177-186

Download Full-Text

View:

Download:

Cites:

Information Journal Paper

Title

Analysis of the Expression of Dopamine Receptors, DRD1 and DRD5, in Cancerous Tissue of Patients with Breast Cancer and the Impact of Quercetin on the Expression of These Receptors in Hs578t and HDF Cell Lines

Author(s)

Kiani Rad Kowsar | RIGI GARSHASB | REIISI SOMAYEH | Issue Writer Certificate

Keywords

Dopamine receptor

Breast cancer

Quercetin

Gene expression

Hs578t cell line

HDF cell line

Abstract

Background & Objectives: The expression of neurotransmitters during cancer progression is one of the factors to consider. This study aimed to evaluate the possible expression changes in Dopamine receptors (DRD1 and DRD5), in breast tumor tissue compared to healthy adjacent samples, and the effect of Quercetin on the expression of these receptors. Materials & Methods: Expression levels of DRD1 and DRD5 were evaluated in 20 breast tumors and healthy adjacent samples using qPCR. The RNA was extracted from all samples, followed by cDNA synthesis and real-time PCR. In vitro experiment was accomplished on Breast cancer cell line, Hs578t. Cells were treated with Quercetin and evaluated cell viability by MTT assay. DRD1 and DRD5 Gene expression was performed in treatment cells compared to untreated control. Statistical analysis was performed to determine the significance. Results: The results showed that the DRD1 and DRD5 were unregulated in Breast cancer tissue (p<0. 01). For cellular experiment, MTT assays revealed that the Quercetin induced a significant decrease in cell viability and proliferation in dose and time-dependent but it was not seen in a normal cell line (HDF). Hs578t cells showed a significant reduction of DRD5 in response to Quercetin. DRD1 gene downregulation was indicated significant in 72h treatment. Conclusions: The effect of Quercetin on the expression of genes encoding DRD1 and DRD5 showed that this substance reduced the expression of these two genes in treated Hs578t cells compared to untreated cells in the same cell line.

Cites

Investigating The Expression Changes of miR-93-3p, NFATc1 and NFATc3 Genes in Peripheral Blood Mononuclear Cells (PBMC) of Breast Cancer Patients

References

Cite

APA: Copy

Kiani Rad, Kowsar, RIGI, GARSHASB, & REIISI, SOMAYEH. (2022). Analysis of the Expression of Dopamine Receptors, DRD1 and DRD5, in Cancerous Tissue of Patients with Breast Cancer and the Impact of Quercetin on the Expression of These Receptors in Hs578t and HDF Cell Lines. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, 12(2), 177-186. SID. https://sid.ir/paper/1001316/en

Vancouver: Copy

Kiani Rad Kowsar, RIGI GARSHASB, REIISI SOMAYEH. Analysis of the Expression of Dopamine Receptors, DRD1 and DRD5, in Cancerous Tissue of Patients with Breast Cancer and the Impact of Quercetin on the Expression of These Receptors in Hs578t and HDF Cell Lines. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES[Internet]. 2022;12(2):177-186. Available from: https://sid.ir/paper/1001316/en

IEEE: Copy

Kowsar Kiani Rad, GARSHASB RIGI, and SOMAYEH REIISI, “Analysis of the Expression of Dopamine Receptors, DRD1 and DRD5, in Cancerous Tissue of Patients with Breast Cancer and the Impact of Quercetin on the Expression of These Receptors in Hs578t and HDF Cell Lines,” JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, vol. 12, no. 2, pp. 177–186, 2022, [Online]. Available: https://sid.ir/paper/1001316/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops