IMMUNOPHENOTYPIC EVALUATION OF PERIPHERAL BLOOD LYMPHOCYTE SUBGROUPS IN PATIENTS WITH ALLERGIC CONTACT DERMATITIS COMPARED WITH OTHER DERMATITIS AND NORMAL INDIVIDUALS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SALAK MOGHADAM A.R.; OSATI ASHTIYANI F.; FIROUZ A.R.; DANESH PAZHOUH MARYAM; DOULATI Y.; ALAEI P.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Razi Journal of Medical Sciences Year:2001 | Volume:7 | Issue:22 Page(s): 272-278

Download Full-Text

Persian Verion

View:

1,531

Download:

Cites:

Information Journal Paper

Title

IMMUNOPHENOTYPIC EVALUATION OF PERIPHERAL BLOOD LYMPHOCYTE SUBGROUPS IN PATIENTS WITH ALLERGIC CONTACT DERMATITIS COMPARED WITH OTHER DERMATITIS AND NORMAL INDIVIDUALS

Author(s)

Keywords

ALLERGIC CONTACT DERMATITISQ4

IRRITABLE CONTACT DCRMATITIS (ICD)Q4

ATOPIC DERMATITIS (AD)Q2

CELL MEDIATED IMMUNITY (CMI)Q3

PATCH TESTQ4

Abstract

Contact dermatitis is seen n two forms of ALLERGIC CONTACT DERMATITIS (ACD) with 20% and irritant contact dermatitis (ICD)with 80% frequency.This disease is manifested in three forms of acute, sub-acute and chronic, characterizes by pain, itching, vesicle, swelling, papul, lichenification and oozing.ACD or contact hypersensitivity (CHS) in an eczematous dermatitis which is regarded as T-cell mediated delayed-type hypersensitivity (DTH) reaction, caused by exposure to some substances act as haptenic allergens.The role of T- Lymphocyte subsets has been clearly shown in the pathogenesis of this disease, However, results from different studies on blood samples are controversial in this regard. This project aimed at a broader study on distribution of different lymphocyte subsets in peripheral blood of patients with CHS compared with other types of dermatitis and normal individuals.Patch - Test is generally required to identify the specific allergen(s). In this study, we used Europen Srand Mdpatch test kit (Trolab) with 23 allergens, tested 46 patients and 36 control healthy individuals. Forty - Eight hours later, 5 ml peripheral blood sample was obtained from the patient and control groups.Serum total IgE of patient and control groups with ELISA were assessed.Peripheral blood samples were stained with Abs (IMK-Plus Kit) Conjugated with FITC and PE, such as; CD 14/45, (CD3+, CD4+,CD8+)T, (CD 16+/ CD56+) NK, (HLA- DR+) T and (CD19+) B, and analyzedon FACS-Caliber (Becton Dickinson Company) Flowcytometry.The results of patch-Test revealed that the most frequent positive reactions were due to nickel - sulphate, cobalt choride, potassium dichromat and formaldehyde.The results of serum total IgE in ACD and ICD showed non significant difference from control group. In Patients with atopic dermatitis total IgE was 8 to 10 times greater than serum IgE in control group (P<0.001).Mean percent, total T-cells (CD3+) and (CD4+) T-cell subset showed non significant change compared to control group (CD8l T-cell subsets showed some increase and total B-cells (CD19+) some decrease which were no significant.CD4/CD8 ratio significantly decreased in ACD patients, but showed significant increase in AD patients with Pvalue of < 0.05.As opposed to total T-Lymphocytes, NK cells (CD 16+/56+) and (HLA-DR+) T-Cells increased significantly (P<0.05).Total number of WBC significantly increased in ACD group compared to control group (P<0.05). In addition, mean percent eosinophil in AD group showed significant increase (P<0.05), comparing with control Individuals. The results of this study indicate the involvement of cell -mediated immunity, specially activated T-cells and increased NK cells in this disease.

Cites

No record.

References

No record.

Cite

APA: Copy

SALAK MOGHADAM, A.R., OSATI ASHTIYANI, F., FIROUZ, A.R., DANESH PAZHOUH, MARYAM, DOULATI, Y., & ALAEI, P.. (2001). IMMUNOPHENOTYPIC EVALUATION OF PERIPHERAL BLOOD LYMPHOCYTE SUBGROUPS IN PATIENTS WITH ALLERGIC CONTACT DERMATITIS COMPARED WITH OTHER DERMATITIS AND NORMAL INDIVIDUALS. RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES), 7(22), 272-278. SID. https://sid.ir/paper/10020/en

Vancouver: Copy

SALAK MOGHADAM A.R., OSATI ASHTIYANI F., FIROUZ A.R., DANESH PAZHOUH MARYAM, DOULATI Y., ALAEI P.. IMMUNOPHENOTYPIC EVALUATION OF PERIPHERAL BLOOD LYMPHOCYTE SUBGROUPS IN PATIENTS WITH ALLERGIC CONTACT DERMATITIS COMPARED WITH OTHER DERMATITIS AND NORMAL INDIVIDUALS. RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES)[Internet]. 2001;7(22):272-278. Available from: https://sid.ir/paper/10020/en

IEEE: Copy

A.R. SALAK MOGHADAM, F. OSATI ASHTIYANI, A.R. FIROUZ, MARYAM DANESH PAZHOUH, Y. DOULATI, and P. ALAEI, “IMMUNOPHENOTYPIC EVALUATION OF PERIPHERAL BLOOD LYMPHOCYTE SUBGROUPS IN PATIENTS WITH ALLERGIC CONTACT DERMATITIS COMPARED WITH OTHER DERMATITIS AND NORMAL INDIVIDUALS,” RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES), vol. 7, no. 22, pp. 272–278, 2001, [Online]. Available: https://sid.ir/paper/10020/en

Related Journal Papers