Design and development of ELISA technique for detection of IgG against Neisseria meningitidis using purification of antisera

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HEJAZI MARYAM; PARYAN MAHDI; Shokri Rahman

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Research in Medicine Year:2022 | Volume:46 | Issue:2 Page(s): 30-39

Download Full-Text

Persian Verion

View:

112

Download:

Cites:

Information Journal Paper

Title

Design and development of ELISA technique for detection of IgG against Neisseria meningitidis using purification of antisera

Author(s)

HEJAZI MARYAM | PARYAN MAHDI | Shokri Rahman | Issue Writer Certificate

Keywords

Meningococcus

Antisera

High purity

ELISA

Tangential Flow Filtration

Abstract

Background and Aim: Neisseria meningitidis is a leading cause of death in children in developed countries. Meningococcus has 13 sero-groups based on the capsule antigens. Rapid diagnosis of the bacteria results in timely selection of the right anti-microbial treatment. The present study, conducted in 2019, aimed to produce a highly pure antiserum for diagnostic assays for the detection of Meningococcus sero-groups. Methods: The type of research study was research and development. Inactivated bacterial suspension was intravenously injected into white New Zealand Rabbits. Serum was collected after the last injection. Purification process was performed using precipitation, chromatography, and Tangential Flow Filtration. The produced antiserum was used in Agglutination and ELISA assays. The purified antiserum had a purity of more than 98% at a suitable quantity. Agglutination test with the primary antigen showed 4+ result. To develop the ELISA assay, the optimized concentration of primary and biotinylated antibodies was 1 and 80 micrograms, respectively. The results of positive predictive value were 100% and negative predictive value was 96%, for the designed assay. Results: The purified antiserum had a purity of more than 98% at a suitable quantity. Agglutination test with the primary antigen showed 4+ result. To developed the ELISA assay, the optimized concentration of primary and biotinylated antibodies was 1 and 80 micrograms, respectively. The results of positive predictive value were 100% and Negative predictive value was 96%, for the designed assay. Conclusion: Serum-based assays are recommended for the timely diagnosis of the disease since these assays are specific, sensitive, inexpensive, and rapid. The antiserum produced in the present research is highly pure. Therefore, we recommend the antiserum for the development of agglutination and ELISA assays for the detection of N. meningitides.

Cites

No record.

References

No record.

Cite

APA: Copy

HEJAZI, MARYAM, PARYAN, MAHDI, & Shokri, Rahman. (2022). Design and development of ELISA technique for detection of IgG against Neisseria meningitidis using purification of antisera. PAJOUHESH DAR PEZESHKI, 46(2 ), 30-39. SID. https://sid.ir/paper/1008707/en

Vancouver: Copy

HEJAZI MARYAM, PARYAN MAHDI, Shokri Rahman. Design and development of ELISA technique for detection of IgG against Neisseria meningitidis using purification of antisera. PAJOUHESH DAR PEZESHKI[Internet]. 2022;46(2 ):30-39. Available from: https://sid.ir/paper/1008707/en

IEEE: Copy

MARYAM HEJAZI, MAHDI PARYAN, and Rahman Shokri, “Design and development of ELISA technique for detection of IgG against Neisseria meningitidis using purification of antisera,” PAJOUHESH DAR PEZESHKI, vol. 46, no. 2 , pp. 30–39, 2022, [Online]. Available: https://sid.ir/paper/1008707/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops