Evaluation and Comparison of PBK and E2F7 Gene Expression Between Early and Advanced Stages of Colorectal Cancer

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HASHEMI REZA; PEYMANI MARYAM; GHAEDI KAMRAN; SAFFAR HANA

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Arak University Medical Sciences Year:2022 | Volume:25 | Issue:1 (155) Page(s): 14-27

Download Full-Text

Persian Verion

View:

101

Download:

Cites:

Information Journal Paper

Title

Evaluation and Comparison of PBK and E2F7 Gene Expression Between Early and Advanced Stages of Colorectal Cancer

Author(s)

HASHEMI REZA | PEYMANI MARYAM | GHAEDI KAMRAN | SAFFAR HANA | Issue Writer Certificate

Keywords

PBK expression

E2F7 expression

Colorectal cancer

ROC curve

StageStage

Abstract

Background and Aim PBK is a mitogen-activated protein kinase (MAPKK) among MEK1/2 and MEK7 and can phosphorylate P38, JNK, and ERK in many cellular functions. The E2F transcription factor family also belongs to a class of cellular regulators acting as oncogenes and tumor suppressors. This study aims to investigate the expression of PBK and E2F7 in the early stages of Colorectal cancer (CRC) compared to advanced stages based on the experimental and TCGA (The Cancer Genome Atlas) database. Methods & Materials A total of 32 tissue samples of patients with CRC with the approval of a pathobiologist were collected according to the examination and criteria reported from different stages. After RNA extraction and cDNA synthesis, the RT-qPCR technique was used to evaluate the expression of the desired genes in the study groups. A receiver operating characteristic (ROC) curve analysis was also used to determine the ability of each of the selected genes to differentiate the two populations: stage I+II and stage III+IV. Ethical Considerations This research was approved by the ethics committee of Islamic Azad University, Shahrekord Branch. (Code IR. IAU. SHK. REC. 1399. 022). Results In this study, it was shown that the expression of PBK and E2F7 significantly increased in stage I+II samples compared to stage III+IV. These data were confirmed by laboratory results and information extracted from the TCGA database. Also, based on the area under curve obtained from the ROC curves, these two genes are significantly distinguishable between stage I+II and III+IV populations in CRC. Conclusion According to the results of this study, PBK and E2F7 genes are good markers in the diagnosis of CRC.

Cites

No record.

References

Cite

APA: Copy

HASHEMI, REZA, PEYMANI, MARYAM, GHAEDI, KAMRAN, & SAFFAR, HANA. (2022). Evaluation and Comparison of PBK and E2F7 Gene Expression Between Early and Advanced Stages of Colorectal Cancer. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 25(1 (155) ), 14-27. SID. https://sid.ir/paper/1008946/en

Vancouver: Copy

HASHEMI REZA, PEYMANI MARYAM, GHAEDI KAMRAN, SAFFAR HANA. Evaluation and Comparison of PBK and E2F7 Gene Expression Between Early and Advanced Stages of Colorectal Cancer. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2022;25(1 (155) ):14-27. Available from: https://sid.ir/paper/1008946/en

IEEE: Copy

REZA HASHEMI, MARYAM PEYMANI, KAMRAN GHAEDI, and HANA SAFFAR, “Evaluation and Comparison of PBK and E2F7 Gene Expression Between Early and Advanced Stages of Colorectal Cancer,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 25, no. 1 (155) , pp. 14–27, 2022, [Online]. Available: https://sid.ir/paper/1008946/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops