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Information Journal Paper

Title

Phytochemical Analysis of Garlic Hydro-alcoholic Extract and Evaluation of its Anti-bacterial Effect on Enterohemorrhagic Escherichia coli in Vitro and ex Vivo

Pages

  25-31

Abstract

 Background and objectives: Garlic is a medicinal plant with various health promoting properties including antimicrobial effects. In this study, we investigated in vitro antibacterial effects of Garlic hydro-alcoholic extract against enterohemorrhagic Escherichia coli (EHEC). Methods: Garlic hydro-alcoholic extract was prepared by maceration method. Phytochemical analysis of the extract was carried out using gas chromatography-mass spectrometry. Minimum inhibitory concentration (MIC) of the extract against EHEC was determined by micro-dilution assay. Cytotoxic effect of the Garlic extract on human colon adenocarcinoma Cell line (SW480) was assessed using MTT assay. Micro-dilution assay was also used to determine the MIC of the extract against EHEC when co-cultured with SW480 cells. Results: The amount of organosulfur in Garlic extract was 70. 91% and the most common organosulfur compounds were trisulfide, di-2-propenyl (34. 8%) and diallyl disulfide (14. 83%). The MIC of Garlic hydro-alcoholic extract on EHEC alone and when co-cultured with SW480 was 12. 5 mg/ml. Concentrations of 12. 5 mg/ml and 25 mg/ml of the extract significantly reduced the viability of SW480 cells compared to control and concentration of 6. 25 mg/ml of Garlic extract (p <0. 0001). Conclusion: The Garlic hydro-alcoholic extract has inhibitory effects on EHEC in vitro. Therefore, it can be considered a suitable candidate for controlling infections caused by EHEC.

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    APA: Copy

    Badeli, Zynab, HAGHKHAH, MASOUD, & GHAEMI, EZZAT ALLAH. (2022). Phytochemical Analysis of Garlic Hydro-alcoholic Extract and Evaluation of its Anti-bacterial Effect on Enterohemorrhagic Escherichia coli in Vitro and ex Vivo. MEDICAL LABORATORY JOURNAL, 16(1), 25-31. SID. https://sid.ir/paper/1014516/en

    Vancouver: Copy

    Badeli Zynab, HAGHKHAH MASOUD, GHAEMI EZZAT ALLAH. Phytochemical Analysis of Garlic Hydro-alcoholic Extract and Evaluation of its Anti-bacterial Effect on Enterohemorrhagic Escherichia coli in Vitro and ex Vivo. MEDICAL LABORATORY JOURNAL[Internet]. 2022;16(1):25-31. Available from: https://sid.ir/paper/1014516/en

    IEEE: Copy

    Zynab Badeli, MASOUD HAGHKHAH, and EZZAT ALLAH GHAEMI, “Phytochemical Analysis of Garlic Hydro-alcoholic Extract and Evaluation of its Anti-bacterial Effect on Enterohemorrhagic Escherichia coli in Vitro and ex Vivo,” MEDICAL LABORATORY JOURNAL, vol. 16, no. 1, pp. 25–31, 2022, [Online]. Available: https://sid.ir/paper/1014516/en

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