Survey of Two Methods Including Phenol-Chloroform and Application of Magnetic Nanoparticles for Genomic DNA Extraction of Staphylococcus aureus

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Rostami Hadiseh; FIROOZEH FARZANEH; ZIBAEI MOHAMMAD; Salahshoorifar Iman; Sobhani Nasab Ali; Nilkbin Vajihe Sadat

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Journal Paper

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Journal: Alborz University Medical Journal Year:2023 | Volume:12 | Issue:1 Page(s): 42-48

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Title

Survey of Two Methods Including Phenol-Chloroform and Application of Magnetic Nanoparticles for Genomic DNA Extraction of Staphylococcus aureus

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Keywords

Staphylococcus aureus

DNA extraction

Magnetic nanoparticles

Phenolchloroform

Bacterial genome

Abstract

Background and Aim: Isolation of genomic DNA from bacterial cells is one of the processes typically performed in most biological laboratories and there are different methods to do it. In this study, two methods including phenol-chloroform, and Magnetic nanoparticles, were used to extract genomic DNA of Staphylococcus aureus. Materials and Methods: In the present study, the standard strain of Staphylococcus aureus ATCC 25923 was used for extraction of genomic DNA by phenol-chloroform and Magnetic nanoparticles methods. Nanodrop and electrophoresis on agarose gel were used to evaluate the quality and concentration of extracted DNA. Results: The concentration of extracted DNA by phenol-chloroform and magnetic nanoparticle) SiO2/Fe3O4) methods were obtained 550. 4 and 131. 6 µ, g/ml respectively. Conclusion: From the findings of this study it can be concluded that due to the thick wall of Staphylococcus aureus, genomic DNA extraction by Magnetic nanoparticles (Fe3O4/SiO2) have acceptable concentration and purity for molecular processes such as PCR, and can be used as an alternative to other extraction methods.

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APA: Copy

Rostami, Hadiseh, FIROOZEH, FARZANEH, ZIBAEI, MOHAMMAD, Salahshoorifar, Iman, Sobhani Nasab, Ali, & Nilkbin, Vajihe Sadat. (2023). Survey of Two Methods Including Phenol-Chloroform and Application of Magnetic Nanoparticles for Genomic DNA Extraction of Staphylococcus aureus. ALBORZ UNIVERSITY MEDICAL JOURNAL, 12(1 ), 42-48. SID. https://sid.ir/paper/1018759/en

Vancouver: Copy

Rostami Hadiseh, FIROOZEH FARZANEH, ZIBAEI MOHAMMAD, Salahshoorifar Iman, Sobhani Nasab Ali, Nilkbin Vajihe Sadat. Survey of Two Methods Including Phenol-Chloroform and Application of Magnetic Nanoparticles for Genomic DNA Extraction of Staphylococcus aureus. ALBORZ UNIVERSITY MEDICAL JOURNAL[Internet]. 2023;12(1 ):42-48. Available from: https://sid.ir/paper/1018759/en

IEEE: Copy

Hadiseh Rostami, FARZANEH FIROOZEH, MOHAMMAD ZIBAEI, Iman Salahshoorifar, Ali Sobhani Nasab, and Vajihe Sadat Nilkbin, “Survey of Two Methods Including Phenol-Chloroform and Application of Magnetic Nanoparticles for Genomic DNA Extraction of Staphylococcus aureus,” ALBORZ UNIVERSITY MEDICAL JOURNAL, vol. 12, no. 1 , pp. 42–48, 2023, [Online]. Available: https://sid.ir/paper/1018759/en

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