Effect of Coenzyme Q10 on DNA Fragmentation, Membrane Integrity, and Sperm Chromatin Condensation after Thawing of Frozen Semen

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

REZAIE MOHAMMAD JAFAR; REZAIE MASOOMEH; NIKKHOO BAHRAM; Roshani Deam; ALLAHVEISI AZRA

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Scientific Journal of Kurdistan University of Medical Sciences Year:2022 | Volume:26 | Issue:7 (117) Page(s): 34-44

Download Full-Text

View:

Download:

Cites:

Information Journal Paper

Title

Effect of Coenzyme Q10 on DNA Fragmentation, Membrane Integrity, and Sperm Chromatin Condensation after Thawing of Frozen Semen

Author(s)

Keywords

Coenzyme Q10

Sperm cryopreservation

Sperm DNA fragmentation

Abstract

Background and Aim: In recent years, Sperm cryopreservation has resulted in significant advances in assisted reproductive techniques (ARTs),and, has showed a crucial potential impact on infertility treatment and increasing success rates of ARTs. The negative effects of sperm freezing due to lack of antioxidants have been well recognized. The aim of this study was to determine the effect of Coenzyme Q10 as a potent antioxidant on sperm parameters including DNA fragmentation, membrane integrity, and sperm chromatin condensation after thawing of frozen semen. Materials and Methods: Semen samples were collected from 50 men referring to the infertility treatment center of Besat Hospital. According to WHO criteria, the men were normozoospermia. Semen samples were divided into three parts: 1) before freezing 2) freezing without Coenzyme Q10, and 3)freezing with Coenzyme Q10. After freezing, samples were stored in liquid nitrogen. After two weeks, the samples were thawed and the parameters of sperm including DNA fragmentation, membrane integrity, and sperm chromatin condensation were evaluated. Data were analyzed by one way ANOVA and Pearson correlation test. Results: After thawing the frozen samples, sperm parameters including number and concentration, total number of motile sperms and progressive movement of sperm, viability, membrane integrity, DNA fragmentation, chromatin density were significantly different from the same parameters before freezing (P <0. 05). Addition of Coenzyme Q10 improved, viability, membrane integrity, chromatin density, and total number of motile sperms and reduced Sperm DNA fragmentation. (P <0. 05) Sperm count and morphology showed no significant difference before and after freezing (P> 0. 05). Also, the percentage of Sperm DNA fragmentation was directly related to sperm chromatin before and after freezing. Conclusion: Addition of Q10 coenzyme antioxidant to sperm freezing medium not only improved sperm parameters of membrane integrity and chromatin, but also resulted in reduced Sperm DNA fragmentation after thawing.

Cites

No record.

References

No record.

Cite

APA: Copy

REZAIE, MOHAMMAD JAFAR, REZAIE, MASOOMEH, NIKKHOO, BAHRAM, Roshani, Deam, & ALLAHVEISI, AZRA. (2022). Effect of Coenzyme Q10 on DNA Fragmentation, Membrane Integrity, and Sperm Chromatin Condensation after Thawing of Frozen Semen. SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES, 26(7 (117) ), 34-44. SID. https://sid.ir/paper/1031654/en

Vancouver: Copy

REZAIE MOHAMMAD JAFAR, REZAIE MASOOMEH, NIKKHOO BAHRAM, Roshani Deam, ALLAHVEISI AZRA. Effect of Coenzyme Q10 on DNA Fragmentation, Membrane Integrity, and Sperm Chromatin Condensation after Thawing of Frozen Semen. SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2022;26(7 (117) ):34-44. Available from: https://sid.ir/paper/1031654/en

IEEE: Copy

MOHAMMAD JAFAR REZAIE, MASOOMEH REZAIE, BAHRAM NIKKHOO, Deam Roshani, and AZRA ALLAHVEISI, “Effect of Coenzyme Q10 on DNA Fragmentation, Membrane Integrity, and Sperm Chromatin Condensation after Thawing of Frozen Semen,” SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES, vol. 26, no. 7 (117) , pp. 34–44, 2022, [Online]. Available: https://sid.ir/paper/1031654/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops