Evaluating Methotrexate Toxicity and Its Association with ABCB1 Genetic Polymorphism in Children with Acute Lymphoblastic Leukemia

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Zakaryaei Farima; MOHAMMADI EBRAHIM; GHADERI EBRAHIM; ZAMANI FATEMEH; Moradveisi Borhan

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Journal Paper

Paper Information

Journal: Iranian Journal of Pediatrics Year:2022 | Volume:32 | Issue:AB0033 Page(s): 0-0

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Title

Evaluating Methotrexate Toxicity and Its Association with ABCB1 Genetic Polymorphism in Children with Acute Lymphoblastic Leukemia

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Keywords

Methotrexate

Acute Lymphoblastic Leukemia

ABCB1 Gene

Drug Complications

Abstract

Background: Acute Lymphoblastic Leukemia (ALL) is among the most prevalent type of hematologic malignancy in children. The Children’, s Oncology Group protocol recognizes Methotrexate (MTX) as a therapy for this problem in children, despite its several complications. The relationship between MTX toxicity and ATP-binding cassette subfamily B member 1 (ABCB1) SNPs in ALL children patients has been investigated in many studies. Objectives: Regarding the controversial findings reported by these studies, the present work aims to evaluate Methotrexate toxicity and its association with ABCB1 Genetic Polymorphism in ALL pediatric patients. Methods: Blood samples were collected from pediatric ALL patients. Next, DNA was extracted and polymerase chain reaction (PCR) was conducted using 300 , Mol/, L of direct primers in 50 , L as the ultimate volume. ABCB1 Gene was amplified using the PCR technique, and 0. 5% agarose gel electrophoresis was used to identify reaction products. Afterward, the PCR fragments’,length was proved by observing through UV-transilluminator. Finally, liver and blood toxicity was studied in all cases under treatment with MTX. Results: In the present study, 81 children with ALL (36 females and 45 males) with a mean age of 6. 32 ,3. 08 years old were examined. The ABCB1 1199 G->A gene mutation frequencyandthe ABCB1 3435 C->T gene mutation frequencywas4. 9and70. 4%, respectively. The results showed no statistically significant difference between leukopenia, gastrointestinal toxicity, renal toxicity, hepatotoxicity, anemia, thrombocytopenia, and neutropenia in cases having homozygous heterozygous ABCB1 3435 C->T and ABCB1 1199 G->A mutant polymorphisms than those having ordinary polymorphism. Conclusions: Overall, it seems that C3435 T, G1199A, and ABCB1 are not significant MTX toxicity markers in pediatric ALL cases.

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APA: Copy

Zakaryaei, Farima, MOHAMMADI, EBRAHIM, GHADERI, EBRAHIM, ZAMANI, FATEMEH, & Moradveisi, Borhan. (2022). Evaluating Methotrexate Toxicity and Its Association with ABCB1 Genetic Polymorphism in Children with Acute Lymphoblastic Leukemia. IRANIAN JOURNAL OF PEDIATRICS, 32(1), 0-0. SID. https://sid.ir/paper/1036047/en

Vancouver: Copy

Zakaryaei Farima, MOHAMMADI EBRAHIM, GHADERI EBRAHIM, ZAMANI FATEMEH, Moradveisi Borhan. Evaluating Methotrexate Toxicity and Its Association with ABCB1 Genetic Polymorphism in Children with Acute Lymphoblastic Leukemia. IRANIAN JOURNAL OF PEDIATRICS[Internet]. 2022;32(1):0-0. Available from: https://sid.ir/paper/1036047/en

IEEE: Copy

Farima Zakaryaei, EBRAHIM MOHAMMADI, EBRAHIM GHADERI, FATEMEH ZAMANI, and Borhan Moradveisi, “Evaluating Methotrexate Toxicity and Its Association with ABCB1 Genetic Polymorphism in Children with Acute Lymphoblastic Leukemia,” IRANIAN JOURNAL OF PEDIATRICS, vol. 32, no. 1, pp. 0–0, 2022, [Online]. Available: https://sid.ir/paper/1036047/en

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