STUDY OF THE ANTIOXIDANT EFFECTS OF TOTAL AND FLAVONOIODS EXTRACTS OF CUSCUTA LEHMANNIANA BUNGE AGAINST ON PC12 CELLS IN VITRO DIABETIC MODEL

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FARHADI MONA; JAMEIE SEYED BEHNAMADDIN; HAYAT PARISA

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Journal: Razi Journal of Medical Sciences Year:2011 | Volume:18 | Issue:90 Page(s): 17-26

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Title

STUDY OF THE ANTIOXIDANT EFFECTS OF TOTAL AND FLAVONOIODS EXTRACTS OF CUSCUTA LEHMANNIANA BUNGE AGAINST ON PC12 CELLS IN VITRO DIABETIC MODEL

Author(s)

FARHADI MONA | JAMEIE SEYED BEHNAMADDIN | HAYAT PARISA | Issue Writer Certificate

Keywords

PC12 CELLSQ2

DIABETIC NEUROPATHYQ2

C. LEHMANIANA BUNGEQ2

Abstract

Background: Central and peripheral neuropathies are the most common side effects of Diabetes Mellitus (DM). The exact mechanisms of diabetic neurotoxicity are still unknown. Recently oxidative stress is introduced as one of the factors for diabetics’ neuropathies. Antioxidants also used as therapeutic agents to reduce the side effects of diabetes. In the present research the antioxidant effects of total and flavonoid extracts of Cusseta lehmaniana Bunge on high glucose inducted Pc12 were studied.Methods: PC12 cell line treated with high glucose was used. Total and flavonoid extract of C. lehmaniana Bungs were prepared. PC12 CELLS treated with 6X fold high glucose concentration exposed to extracts. Antioxidant activity of extracts, cell viability and apoptosis were studied by DPPH, MTT, Annexin staining and Western Blotting respectively.Results: The MTT result showed 50 mg/ml of flavonoid extract and 100 mg/ml of total C. lehmaniana Bungs extract increased cell viability after 3 hours pretreatment. DPPH assay demonstrated 50 mg/ml flavonoid extract can increased radical scavenging inhibits compare with ascorbic acid. Several assays were used for evaluated of anti apoptotic effect such as: Annexin, SubG1and Western blotting for expression of Bax protein.Conclusion: Based on our findings, it is concluded that both total and flavonoid extract of C. lehmaniana Bungs have the potential to protect PC12 CELLS against glucose neurotoxicity by reducing apoptosis via increased Bax expression protein.

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APA: Copy

FARHADI, MONA, JAMEIE, SEYED BEHNAMADDIN, & HAYAT, PARISA. (2011). STUDY OF THE ANTIOXIDANT EFFECTS OF TOTAL AND FLAVONOIODS EXTRACTS OF CUSCUTA LEHMANNIANA BUNGE AGAINST ON PC12 CELLS IN VITRO DIABETIC MODEL. RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES), 18(90), 17-26. SID. https://sid.ir/paper/10600/en

Vancouver: Copy

FARHADI MONA, JAMEIE SEYED BEHNAMADDIN, HAYAT PARISA. STUDY OF THE ANTIOXIDANT EFFECTS OF TOTAL AND FLAVONOIODS EXTRACTS OF CUSCUTA LEHMANNIANA BUNGE AGAINST ON PC12 CELLS IN VITRO DIABETIC MODEL. RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES)[Internet]. 2011;18(90):17-26. Available from: https://sid.ir/paper/10600/en

IEEE: Copy

MONA FARHADI, SEYED BEHNAMADDIN JAMEIE, and PARISA HAYAT, “STUDY OF THE ANTIOXIDANT EFFECTS OF TOTAL AND FLAVONOIODS EXTRACTS OF CUSCUTA LEHMANNIANA BUNGE AGAINST ON PC12 CELLS IN VITRO DIABETIC MODEL,” RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES), vol. 18, no. 90, pp. 17–26, 2011, [Online]. Available: https://sid.ir/paper/10600/en

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