Characterization of the conserved regions of E1A protein from human adenovirus for reinforcement of cytotoxic T lymphocytes responses to the all genogroups causes ocular manifestation through an in silico approach

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OMIDI NAHID; AZARAN AZARAKHSH; MAKVANDI MANOOCHEHR; Khataminia Gholamreza; AHMADI ANGALI KAMBIZ; JALILIAN SHAHRAM

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Journal Paper

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Journal: Iranian Journal of Microbiology Year:2022 | Volume:14 | Issue:5 Page(s): 746-758

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Title

Characterization of the conserved regions of E1A protein from human adenovirus for reinforcement of cytotoxic T lymphocytes responses to the all genogroups causes ocular manifestation through an in silico approach

Author(s)

Keywords

In silico model

Adenovirus E1A proteins

Keratoconjunctivitis

Molecular docking

Cytotoxic T-lymphocyte

Abstract

Background and Objectives: Adenovirus species B, C, D, and E are the most common causes of ocular manifestations caused by adenoviruses. FDA-approved treatment agents for adenovirus infections are not available. Cell-mediated im-munity is the major protective mechanism versus human adenoviruses (HAdVs) infection and T cells specific for peptide epitopes from nonstructural proteins can prevent adenoviral dissemination. E1A CR2 region of HAdVs Epitopes predicted for reinforcing cytotoxic T lymphocytes (CTLs) in the EKC patients. Among human adenoviruses E1 protein, four distinct E1A regions had a significantly higher level of homology than the rest of E1A protein. E1A protein inhibits IFN signal trans-duction. Epitope-based vaccines are designed to have flexible and simple methods to synthesize a vaccine, using an adjuvant to trigger fast immune responses. CTL epitopes were applied to create a multiepitope vaccine. Conserve region1 (CR1) and CR3 have less antigenicity compared to CR2. Additionally, CR3 in HAdV-D8 contains three toxic areas. CR4 similar to the two regions CR1 and CR3 do not show acceptable antigenic properties. Materials and Methods: Bioinformatics’,tools were used to predict, refine and validate the 3D structure of the construct. Effective binding was predicted by protein-protein docking of the epitope vaccine with MHC-I molecules and revealed the safety and efficacy of the predicted vaccine construct. Results: In silico analysis show that rising levels of cytotoxic CD8 + T cells, TH1 cells, macrophages, and neutrophils are linked to IFN-dominant TH1-type responses, which are detected in putative immune individuals. Conclusion: Combined with 3D protein modeling, this study predicted the epitopes of E1A CR2 protein in HAdVs.

Cites

Genomic Analysis of E1A and E1B Regions of Human Adenovirus Type 8 in Patients with Adenoviral Keratoconjunctivitis in Ahvaz City

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APA: Copy

OMIDI, NAHID, AZARAN, AZARAKHSH, MAKVANDI, MANOOCHEHR, Khataminia, Gholamreza, AHMADI ANGALI, KAMBIZ, & JALILIAN, SHAHRAM. (2022). Characterization of the conserved regions of E1A protein from human adenovirus for reinforcement of cytotoxic T lymphocytes responses to the all genogroups causes ocular manifestation through an in silico approach. IRANIAN JOURNAL OF MICROBIOLOGY, 14(5), 746-758. SID. https://sid.ir/paper/1074710/en

Vancouver: Copy

OMIDI NAHID, AZARAN AZARAKHSH, MAKVANDI MANOOCHEHR, Khataminia Gholamreza, AHMADI ANGALI KAMBIZ, JALILIAN SHAHRAM. Characterization of the conserved regions of E1A protein from human adenovirus for reinforcement of cytotoxic T lymphocytes responses to the all genogroups causes ocular manifestation through an in silico approach. IRANIAN JOURNAL OF MICROBIOLOGY[Internet]. 2022;14(5):746-758. Available from: https://sid.ir/paper/1074710/en

IEEE: Copy

NAHID OMIDI, AZARAKHSH AZARAN, MANOOCHEHR MAKVANDI, Gholamreza Khataminia, KAMBIZ AHMADI ANGALI, and SHAHRAM JALILIAN, “Characterization of the conserved regions of E1A protein from human adenovirus for reinforcement of cytotoxic T lymphocytes responses to the all genogroups causes ocular manifestation through an in silico approach,” IRANIAN JOURNAL OF MICROBIOLOGY, vol. 14, no. 5, pp. 746–758, 2022, [Online]. Available: https://sid.ir/paper/1074710/en

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