IN VITRO ASSESSMENT OF CHLOROHEXIDINE AND CALCIUM HYDROXIDE PASTE WITH CHLOROHEXIDINE AND SALINE SOLUTIONS AGAINST E- FEACALIS

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ZARE JAHROMI M.; ASNA ASHARI ESFAHANI MASIH

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Journal: Journal of Dentistry Year:2009 | Volume:10 | Issue:2 (23) Page(s): 99-104

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Title

IN VITRO ASSESSMENT OF CHLOROHEXIDINE AND CALCIUM HYDROXIDE PASTE WITH CHLOROHEXIDINE AND SALINE SOLUTIONS AGAINST E- FEACALIS

Author(s)

ZARE JAHROMI M. | ASNA ASHARI ESFAHANI MASIH | Issue Writer Certificate

Keywords

CHLORHEXIDINEQ2

CALCIUM HYDROXIDEQ2

ENTEROCOCUS FEACALISQ2

Abstract

Statement of Problem: The use of CALCIUM HYDROXIDE powder in saline as an intracanal drug iscommon. Recently, the use of CHLORHEXIDINE solution for irrigation due to its antibacterial property has increased significantly. Purpose: The Purpose of this study was to compare the antimicrobial activity of CALCIUM HYDROXIDE combined with CHLORHEXIDINE gluconate against E-Feacalis and to compare the results with the CALCIUM HYDROXIDE mixed with saline and by CHLORHEXIDINE alone.Materials and Methods: In this study, 40 teeth with single canal were used. After removing the crown and preparation of the canal with step-back technique, the root canal was irrigated with EDTA solution to remove smear layer. Then, all the samples were sterilized in autoclave and the roots were infected with E faecalis and incubated. Subsequently, the roots were divided into 3 treatment groups. Group 1 was treated with calcium powder hydroxide in salin, group 2 with CALCIUM HYDROXIDE powder in CHLORHEXIDINE, and group 3 with CHLORHEXIDINE. All the samples were incubated for a period of one week at 37°C. Sampling was done by paper point. The microbiological samples were plated to count the colony-forming units and the level of CFU was assessed at the wavelength of 540nm by photometer. The mean number of colonies in all the three groups was assessed by variance analysis, and turbidity and transparency of the samples were assessed via chi-squares (x2) test.Results: The results showed that CHLORHEXIDINE gel was significantly more effective than CALCIUM HYDROXIDE with CHLORHEXIDINE, CALCIUM HYDROXIDE, and control saline solution (p=0.06).Conclusion: The results revealed that CHLORHEXIDINE gel has antibacterial effects against E-Feacalis. In fact, the study in the field showed that adding CHLORHEXIDINE to CALCIUM HYDROXIDE results in an increase in antibacterial effect of CALCIUM HYDROXIDE and reduces the antimicrobial effect of CHLORHEXIDINE.

Cites

COMPARING THE ANTIMICROBIAL EFFECTS EFFICACY OF MTAD, CHLORHEXIDINE AND SODIUM HYPOCHLORITE ON AEROBIC MICROORGANISMS IN NECROTIC ROOT CANALS: AN INVIVO STUDY

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APA: Copy

ZARE JAHROMI, M., & ASNA ASHARI ESFAHANI, MASIH. (2009). IN VITRO ASSESSMENT OF CHLOROHEXIDINE AND CALCIUM HYDROXIDE PASTE WITH CHLOROHEXIDINE AND SALINE SOLUTIONS AGAINST E- FEACALIS. JOURNAL OF DENTISTRY (SHIRAZ UNIVERSITY OF MEDICAL SCIENCES), 10(2 (23)), 99-104. SID. https://sid.ir/paper/108412/en

Vancouver: Copy

ZARE JAHROMI M., ASNA ASHARI ESFAHANI MASIH. IN VITRO ASSESSMENT OF CHLOROHEXIDINE AND CALCIUM HYDROXIDE PASTE WITH CHLOROHEXIDINE AND SALINE SOLUTIONS AGAINST E- FEACALIS. JOURNAL OF DENTISTRY (SHIRAZ UNIVERSITY OF MEDICAL SCIENCES)[Internet]. 2009;10(2 (23)):99-104. Available from: https://sid.ir/paper/108412/en

IEEE: Copy

M. ZARE JAHROMI, and MASIH ASNA ASHARI ESFAHANI, “IN VITRO ASSESSMENT OF CHLOROHEXIDINE AND CALCIUM HYDROXIDE PASTE WITH CHLOROHEXIDINE AND SALINE SOLUTIONS AGAINST E- FEACALIS,” JOURNAL OF DENTISTRY (SHIRAZ UNIVERSITY OF MEDICAL SCIENCES), vol. 10, no. 2 (23), pp. 99–104, 2009, [Online]. Available: https://sid.ir/paper/108412/en

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