An in vitro investigation of the apoptosis-inducing activity of corosolic acid in breast cancer cells

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Abdalkareem Jasim Saade; Zedan Khalaf Omer; Hamoud Alshahrani Shadia; Hachem Kadda; Ziyadullaev Shukhrat; Turki Jalil Abduladheem; Wang ChangMing; S. Zabibah Rahman; A. Bin Jardan Yousef; A. Qasim Qutaiba; Suliman Maashi Marwah; Fakri Mustafa Yasser

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Journal: Iranian Journal of Basic Medical Sciences Year:2023 | Volume:26 | Issue:4 Page(s): 453-460

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Title

An in vitro investigation of the apoptosis-inducing activity of corosolic acid in breast cancer cells

Author(s)

Keywords

ApoptosisQ4

Breast cancerQ4

Corosolic acidQ4

JAK2Q4

STAT3Q4

Abstract

Objective(s): Breast cancer is the most prevalent cancer among females with different molecular subtypes. Corosolic acid is a pentacyclic triterpenoid with anti-cancer properties. Materials and Methods: The MTT assay was used to assess the cytotoxic activity of Corosolic acid on MDA-MB-231 and MCF7 cell lines. To determine the apoptotic cells, the flow cytometry technique was utilized. The expression levels of Apoptosis-related genes and proteins were quantified using quantitative real time-PCR (qRT-PCR) and Western blotting methods. The activity of caspase enzymes was measured by spectrophotometry. Results: Corosolic acid significantly inhibited the proliferation of both cell lines compared with controls. This agent markedly induced Apoptosis in MDA-MB-231 cells but did not affect MCF7 cells compared with controls. Treating the MADA-MB-231 and MCF7 cell lines with Corosolic acid showed an inducing effect on Apoptosis-associated caspases, including Caspase-8, 9, and-3, in MADA-MB-231 cells with no effect on apoptotic markers in MCF7 cells. Further experiments uncovered Corosolic acid-induced Apoptosis in MADA-MB-231 cells by decreasing the expression of the phosphorylated form of JAK2 and STAT3 proteins. Conclusion: The present data suggested that Corosolic acid is an Apoptosis-inducing phytochemical in triple-negative Breast cancer MADA-MB-231 cells. Also, Corosolic acid triggered Apoptosis in these cells by stimulating both pathways of Apoptosis and inhibiting the JAK/STAT signaling. Furthermore, Corosolic acid was found to inhibit MCF7 cell proliferation by a non-apoptotic mechanism.

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APA: Copy

Abdalkareem Jasim, Saade, Zedan Khalaf, Omer, Hamoud Alshahrani, Shadia, Hachem, Kadda, Ziyadullaev, Shukhrat, Turki Jalil, Abduladheem, Wang, ChangMing, S. Zabibah, Rahman, A. Bin Jardan, Yousef, A. Qasim, Qutaiba, Suliman Maashi, Marwah, & Fakri Mustafa, Yasser. (2023). An in vitro investigation of the apoptosis-inducing activity of corosolic acid in breast cancer cells. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 26(4), 453-460. SID. https://sid.ir/paper/1085309/en

Vancouver: Copy

Abdalkareem Jasim Saade, Zedan Khalaf Omer, Hamoud Alshahrani Shadia, Hachem Kadda, Ziyadullaev Shukhrat, Turki Jalil Abduladheem, Wang ChangMing, S. Zabibah Rahman, A. Bin Jardan Yousef, A. Qasim Qutaiba, Suliman Maashi Marwah, Fakri Mustafa Yasser. An in vitro investigation of the apoptosis-inducing activity of corosolic acid in breast cancer cells. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2023;26(4):453-460. Available from: https://sid.ir/paper/1085309/en

IEEE: Copy

Saade Abdalkareem Jasim, Omer Zedan Khalaf, Shadia Hamoud Alshahrani, Kadda Hachem, Shukhrat Ziyadullaev, Abduladheem Turki Jalil, ChangMing Wang, Rahman S. Zabibah, Yousef A. Bin Jardan, Qutaiba A. Qasim, Marwah Suliman Maashi, and Yasser Fakri Mustafa, “An in vitro investigation of the apoptosis-inducing activity of corosolic acid in breast cancer cells,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 26, no. 4, pp. 453–460, 2023, [Online]. Available: https://sid.ir/paper/1085309/en

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