Evaluation of the Protective Effect of Human Platelet-Rich Plasma on Spermatogenesis and Sperm Chromatin Condensation Following Ischemia-Reperfusion Injury Due to Spermatic Cord Torsion in Mice

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Ramesh Mahya; KHADIVI FARNAZ

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Shahid Sadoughi University of Medical Sciences Year:2023 | Volume:30 | Issue:12 Page(s): 6211-6222

Download Full-Text

Persian Verion

View:

Download:

Cites:

Information Journal Paper

Title

Evaluation of the Protective Effect of Human Platelet-Rich Plasma on Spermatogenesis and Sperm Chromatin Condensation Following Ischemia-Reperfusion Injury Due to Spermatic Cord Torsion in Mice

Author(s)

Ramesh Mahya | KHADIVI FARNAZ | Issue Writer Certificate

Keywords

Spermatic cord torsion

Platelet-rich plasma

Spermatogenesis

chromatin

Abstract

Introduction: Torsion of spermatic cord induces ischemic-reperfusion injury which leads to testicular dysfunction, apoptosis of germ cells, ROS generation and infertility. Platelet-rich plasma (PRP) is a rich source of growth factors and anti-inflammatory cytokines. In this study, the effects of PRP on Spermatogenesis and sperm chromatin condensation after ischemia-reperfusion injury were evaluated. Methods: Forty adult male mice were procured in the study. Control group, torsion/ detorsion group, negative control group: torsion/ detorsion surgery with intra testicular injection of culture medium, treatment group: torsion/ detorsion surgery with intra testicular injection of PRP. After one cycle of Spermatogenesis, histomorphometric evaluation was performed by PAS staining and sperm chromatin condensation was assessed by aniline blue staining. Data was analyzed by using SPSS Statistics and ANOVA followed by Tukey test. Results: The mean number of spermatogenic cells, Sertoli, Leydig, Myoid, Johnson score and mean diameter and thickness of germinal epithelium were significantly decreased following ischemia-reperfusion injury. Mean thickness of seminiferous tubules basement membrane as well as sperm percentage with residual histone increased after ischemia-reperfusion injury (P <0. 001). After PRP treatment, there was a significant improvement in the histological structure of the tubules, cell viability, and sperm chromatin condensation (P <0. 001). While, a significant difference was observed in comparison with control group. Conclusion: PRP can partially ameliorate the complications of ischemia-reperfusion injury through its growth factors and anti-inflammatory cytokines involved in angiogenesis and antioxidant activity.

Cites

No record.

References

Cite

APA: Copy

Ramesh, Mahya, & KHADIVI, FARNAZ. (2023). Evaluation of the Protective Effect of Human Platelet-Rich Plasma on Spermatogenesis and Sperm Chromatin Condensation Following Ischemia-Reperfusion Injury Due to Spermatic Cord Torsion in Mice. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 30(12 ), 6211-6222. SID. https://sid.ir/paper/1087116/en

Vancouver: Copy

Ramesh Mahya, KHADIVI FARNAZ. Evaluation of the Protective Effect of Human Platelet-Rich Plasma on Spermatogenesis and Sperm Chromatin Condensation Following Ischemia-Reperfusion Injury Due to Spermatic Cord Torsion in Mice. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2023;30(12 ):6211-6222. Available from: https://sid.ir/paper/1087116/en

IEEE: Copy

Mahya Ramesh, and FARNAZ KHADIVI, “Evaluation of the Protective Effect of Human Platelet-Rich Plasma on Spermatogenesis and Sperm Chromatin Condensation Following Ischemia-Reperfusion Injury Due to Spermatic Cord Torsion in Mice,” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 30, no. 12 , pp. 6211–6222, 2023, [Online]. Available: https://sid.ir/paper/1087116/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops