Treatment with conditioned culture media with human mesenchymal stem cells on reserpine-induced thermal fibromyalgia model in rats

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Jasbinia Farzaneh; HASSANPOUREZATTI MAJID

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Journal Paper

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Journal: Journal of Birjand University of Medical Sciences Year:2022 | Volume:29 | Issue:3 Page(s): 218-228

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Information Journal Paper

Title

Treatment with conditioned culture media with human mesenchymal stem cells on reserpine-induced thermal fibromyalgia model in rats

Author(s)

Jasbinia Farzaneh | HASSANPOUREZATTI MAJID | Issue Writer Certificate

Keywords

Culture Medium

Hippocampus

Inflammation

Mesenchymal Stem Cells

Pain

Reserpine

Abstract

Background and Aims: Fibromyalgia (FM) is a chronic Pain syndrome affected by the hippocampal function. Mesenchymal Stem Cells-conditioned medium (MSC-CMs) has shown antioxidant and neuroprotective potentials in treating various diseases. The present study aimed to investigate the efficacy of MSC-CMs on the thermal treatment of Reserpine-induced FM and evaluate the concentration changes of tumor necrosis factor-alpha (TNF-α, ) in the Hippocampus of rats under these conditions. Materials and Methods: 220 g. Animals received Reserpine (1 mg/kg) in the FM groups, and subcutaneous injection of normal saline in other groups, once a day for 3 consecutive days. Then, the rats received MSC-CM (0. 3 and 0. 5 mg/ml protein content, intraperitoneally) once a week for three weeks. The thermal Pain threshold of mice was evaluated using tail flick and hot plate tests at the beginning and end of the experiments. TNF-α,concentration in the Hippocampus was determined by an ELISA kit at the end of the experimental period. Results: The thermal Pain threshold of rats in the FM group decreased significantly (P<0. 01) in both Pain tests compared to that of the control group. A significant increase (P<0. 01) was observed in the level of TNF-α,in the Hippocampus of rats in the FM group compared to that of the control group. Treatment with MSC-CMs significantly reduced Reserpine-induced Pain and hippocampal TNF-a concentration despite the effect of fresh and conditioned medium on control rats. Conclusion: Treatment with MSC-CMs could control thermal Pain in the rat model of Reserpine-induced fibromyalgia by controlling the TNF-a level in the Hippocampus.

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APA: Copy

Jasbinia, Farzaneh, & HASSANPOUREZATTI, MAJID. (2022). Treatment with conditioned culture media with human mesenchymal stem cells on reserpine-induced thermal fibromyalgia model in rats. JOURNAL OF BIRJAND UNIVERSITY OF MEDICAL SCIENCES, 29(3 ), 218-228. SID. https://sid.ir/paper/1092616/en

Vancouver: Copy

Jasbinia Farzaneh, HASSANPOUREZATTI MAJID. Treatment with conditioned culture media with human mesenchymal stem cells on reserpine-induced thermal fibromyalgia model in rats. JOURNAL OF BIRJAND UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2022;29(3 ):218-228. Available from: https://sid.ir/paper/1092616/en

IEEE: Copy

Farzaneh Jasbinia, and MAJID HASSANPOUREZATTI, “Treatment with conditioned culture media with human mesenchymal stem cells on reserpine-induced thermal fibromyalgia model in rats,” JOURNAL OF BIRJAND UNIVERSITY OF MEDICAL SCIENCES, vol. 29, no. 3 , pp. 218–228, 2022, [Online]. Available: https://sid.ir/paper/1092616/en

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